Job Description

Payroll Title:
SRA 1
Department:
PEDIATRICS/Genetics
Salary Range
Commensurate with Experience
Worksite:
Campus
Appointment Type:
Career
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
1
Work Schedule:
Days, 8 hour shifts, Monday-Friday

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#119858 Stem Cell Research Technician

Filing Deadline: Wed 12/7/2022

UC San Diego values equity, diversity, and inclusion. If you are interested in being part of our team, possess the needed licensure and certifications, and feel that you have most of the qualifications and/or transferable skills for a job opening, we strongly encourage you to apply.

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy

UCSD Layoff from Career Appointment: Apply by 11/04/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 11/15/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.

DESCRIPTION

The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined, and are integral to the goals of the department.

The Department has undergone significant growth in recent years with a consolidated budget of approximately $106 million including sponsored projects expenditures of approximately $54 million and clinical revenue of over $ 52 million. This expansion of the Department’s research and clinical portfolio is expected to continue in the next few years.

The Department’s 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.

In 2001, physicians and leadership of the University of California, San Diego (UCSD), Rady Children's, and Children's Specialists of San Diego (CSSD) unified pediatric patient care, research, education and community service programs, creating a university-affiliated children's health system to serve the region. Over the past two decades, an extensive list of joint programs has developed, with many physicians and researchers playing a role at both institutions.

This affiliation has consolidated the clinical, teaching, research and public service programs of the UCSD School of Medicine's Department of Pediatrics with San Diego's only health care system dedicated to the health and well-being of children. In 2009, this partnership was furthered by the creation of Rady Children’s Specialists of San Diego, a Medical Foundation, and is now recognized as having global leaders in research, technology, translational medicine, education and clinical excellence.

Assist with and perform standard laboratory procedures with Human Pluripotent Stem Cells (hESC/iPSC) and develop brain organoids as well as other experimental procedures. Incumbent will assist PIs and other lab members with studies. Studies will include: 1) General growth, expansion, maintenance, population enrichment, and induction of specific neural lines from stem cells. Induction of neural lines will involve the generation of: human astrocytes (using 3D neurosphere culture techniques), organoids, and human neurons. 2) Gene manipulation of stem cells with silencing or overexpression viral vectors constructs. 3) Characterization of resultant phenotypes (by molecular, multi-electrode array, and imaging techniques). 4) Molecular biology techniques involving the isolation of RNA and protein to quantify gene and protein expression. Procedures will include molecular biology (generation of transgene constructs, PCR, RNA isolation and quantification), histology, light microscopy of embryonic and neuronal cell lines as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, immunohistochemistry, Western blot, ELISA, tissue culture and maintenance of biochemistry equipment). Perform routine tissue culture of primary cells, preparation of lentiviruses and viral infection techniques. The candidate will be responsible for oral and written reports every other week.

MINIMUM QUALIFICATIONS

  • Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell, or related field.

  • Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis.

  • Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions.

  • Experience with general lab maintenance including cleaning, stocking supplies.

  • Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel.

  • Ability to follow step-based experimental protocols.

  • Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.

  • Ability to prioritize and perform efficiently.

  • Skill and ability to keep accurate data.

  • Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.

PREFERRED QUALIFICATIONS

  • Experience with UCSD policies and procedures and basic safety rules and issues.

SPECIAL CONDITIONS

  • Employment is subject to a criminal background check.
  • Willingness to work with animals, viral vectors, radioactive material, human primary tissue and standard hazardous laboratory compounds.
  • Willing to work evenings and weekends when necessary.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

Application Instructions

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