DESCRIPTION

The UCSD Department of Pharmacology is one of two basic science units in the School of Medicine where world-class researchers investigate the effects of chemical interventions on biological systems. The faculty are internationally renowned for their strength in elucidating basic cellular and molecular mechanisms of signal transduction that lead to innovative drug target identification and ultimately to cutting edge drug discovery. The faculty also plays a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines, and provide diversity to meet the interests of a broad spectrum of students and scholars. More than one hundred trainees at the graduate student and postdoctoral level, as well as a staff of more than 50 research technicians and department administrators interact closely with the faculty. The diverse mix of ages, backgrounds, and talents creates a robust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced and interested individuals to become a part of our dynamic exploratory research and educational environment. For more information, please visit our website at http://pharmacology.ucsd.edu.

The Staff Research Associate 2 position will work in the Idoyaga Lab, whose primary focus is to understand the functional specialization of innate immune cells (dendritic cells, monocytes and macrophages) in health and disease, and to design novel therapeutic strategies that harness the power of these cells in establishing immune responses. The Idoyaga lab uses samples obtained from patients, as well as different mouse models, to evaluate the mechanisms that these innate cells are using to initiate and modulate immune responses. In particular, the incumbent will be involved in characterizing innate immune cells in healthy gut, and their changes in a gastrointestinal disease named gastroparesis. Under general supervision, the SRA2 will perform a wide variety of experimental research techniques related to human and mouse immunology including digestion of tissues, operation of a cell sorter and flow cytometer, operation of a fluorescent microscope, and analyze data, arrive to conclusions and generate hypothesis for the projects to move forward. The SRA 2 will also be involved in laboratory management activities including inventory, ordering supplies, maintaining equipment and ensure that EH&S regulations are met. Finally, the SRA2 will be expected to participate in all the intellectual activities of the laboratory, including group meetings, manuscript discussions and seminar.

MINIMUM QUALIFICATIONS

• Theoretical knowledge in biology, chemistry and/or biochemistry, typically attained with a Bachelor’s degree or equivalent combination of education, training and experience.

• Knowledge and experience with basic laboratory procedures and equipment, including sterile techniques, preparation of buffers or cell culture.

• Knowledge of immunology and other demonstrated equivalent experience. BS/BA in immunology, biological sciences, or related field or an equivalent combination of education and experience.

• Experience in preparing chemical solutions.

• Knowledge of various biological and immunological techniques such as cell culture, in vitro experimentation and immunodetection techniques such as ELISA.

• Knowledge of or experience with flow cytometry and their data analysis.

• Knowledge of or experience with a broad range of standard molecular biology techniques such as DNA and RNA extraction, PCR, RT-PCR, electrophoresis, etc.

• Knowledge of or experience with mammalian cellular biology techniques and mammalian cell culture.

• Knowledge of or experience with light and fluorescence microscopy.

• Knowledge of or experience with preparation of libraries for sequencing.

• Demonstrated ability to be meticulous in the maintenance of detailed computerized records such as experimental records, inventory, and facility usage.

• General knowledge of solubility, general laboratory techniques, and scientific calculations sufficient to prepare and maintain complex chemical stocks such as dilutions, buffers, reagents, and solutions.

• Excellent interpersonal skills to communicate in person, on the phone, and in writing to a diverse population including persons from foreign countries, the business community, academicians, students, and staff with a high degree ofdiplomacy, mature judgment, tact, and flexibility.

• Experience using computerized office software such as Internet Explorer, PowerPoint, Word, Excel, Adobe Photoshop, Reader, Illustrator, and Acrobat. Experience using computerized analysis software, statistics programs, gel documentation/analysis programs, and provide assistance as needed.

• Skill in working in a fast paced, research environment with frequent interruptions while completing projects and work assignments in a timely and efficient manner. Ability to organize, prioritize, and meet deadlines.

• Ability to learn and understand UCSD policies, ability to provide work direction, oversee and train part-time student workers.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.

• Willingness to work evenings, if necessary.

• Willingness to work with animals and human primary samples.