DESCRIPTION

The incumbent will design and implement research protocols and conduct experiments for ongoing research projects in the laboratory. Responsibilities include conducting mechanical design of shear-stress inducing flow system for vascular cells, molecular biological experiments to understand the mechano-transduction mechanism in vascular disease pathogenesis and therapy.

Emphasis will be on spatial genomic and proteomic analysis of endothelial cells in 3D-printed vascular models such as aneurysm perfusion system. The incumbent will perform experimentation design and implementation, data acquisition and analysis, grant and manuscript preparation, and presentation of findings at lab meetings and conferences. Additional responsibilities include training lab members, graduate, medical and undergraduate students.

~ This position is eligible for a flexible work arrangement, which could include a non-standard work schedule, and/or the ability to work off-site part of the time. The employee shall be available full-time during assigned work hours (specific hours to be discussed with supervisor).

QUALIFICATIONS

• Strong theoretical and practical knowledge in advanced molecular biology and cell physiology attained through a Bachelors degree in biochemistry, bioengineering, or related field OR an equivalent combination of education and laboratory experience.

• Advanced knowledge of Aseptic techniques for cell/tissue culture and animal handling in a laboratory.

• Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.

• Demonstrated experience assisting with writing scientific manuscripts and contributing to teamwork in molecular biological experiments.

• Demonstrated ability and experience to isolate heart and lung vascular endothelial and smooth muscle cells and prepare primary cultured cells for molecular biological and cell physiological experiments.

• Demonstrated ability and experience in gene cloning and silencing, production of Adeno- and Adeno-associated virus to transduce genes in the cells and tissues.

• Demonstrated ability and experience in Western Blot, immunoprecipitation, site-directed mutagenesis, PCR, qPCR, and single-cell RT-PCR.

• Demonstrated ability and experience in electrophysiological experiments and immunofluorescence experiments using a fluorescence microscope.

• Demonstrated skill to work with animals, as well as animal and human tissues.

• Demonstrated knowledge of the following specialized programs; SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.

• Demonstrated ability to read scientific literature, understand appropriate study design, follow study procedures and protocol, and maintain participant confidentiality.

• Demonstrated effective time management skills to plan and organize work assignments in an efficient and productive manner.

• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

• Effective and diplomatic oral and written communication and interpersonal skills. Ability to work with a variety of personnel from different laboratories and instruct in optimal procedures for assays.

SPECIAL CONDITIONS

• Must be available to work some weekends & evenings to complete experiments.

• Must be able to work with laboratory animals (mice).

• Job offer is contingent upon satisfactory clearance based on Background Check results.

• The Institute for Engineering Medicine (IEM) at University of California, San Diego, is committed to actively promoting our Principles of Community and continually advancing equity, diversity, and inclusion within all levels of our organization. In line with the goals of UC San Diego's Strategic Plan for Inclusive Excellence supporting a diverse faculty, staff, and student body and fostering a positive and welcome climate where all are valued, we strongly encourage candidates from underrepresented communities, to apply.