DESCRIPTION

Under the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures in yeast and mammalian cells. Yeast work will include standard yeast genetic manipulation and culture. Mammalian cell work will also include standard tissue culture cell maintenance and transfection of plasmid DNA.

Incumbent will provide work direction for undergraduate lab assistants and general lab support for graduate students, participate in lab meetings and seminars related to the projects, and perform other techniques as required. This position also encompasses a variety of laboratory maintenance duties that include organizing, record keeping, and managing inventory of all lab supplies and maintenance of lab equipment.

QUALIFICATIONS

• Theoretical and practical knowledge of genetics, molecular biology, and evolution including relevant laboratory techniques, as typically acquired through a BS in Biology and one (1+) or more years of lab experience in any of the related fields, or equivalent combination of education and experience..

• Proven ability to carry out research projects or portions of research projects with direction from PI or other experienced lab members, including (a) reading and comprehending primary scientific literature; (b) independently planning, organizing, performing, and troubleshooting experiments; (c) interpreting and presenting data.

• Experience in molecular cloning, including PCR, gel electrophoresis, DNA cloning, and plasmid preps.

• Experience in yeast and/or bacterial genetics.

• Demonstrated experience in mammalian tissue culture

• Proven knowledge and skill with sterile techniques for bacterial culture.

• Proven experience with phase contrast and fluorescence microscopy.

• Excellent (English) verbal, written, and electronic communication skills that facilitate professional, effective, and courteous interactions with diverse personalities. Skill to work as part of a research team.

• Experience using laboratory equipment (centrifuges, light microscopes, balances, pH meter, incubators, spectrophotometers, and electrophoresis apparatus).

• Skill and knowledge to proactively maintain an organized lab and stocks of essential supplies and media, purchase new equipment, and ensure lab equipment is operating effectively.

• Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Ability to keep safety training current and consistent with research involvement.

• Familiarity with Apple and PC computers and demonstrated ability to work independently with a variety of computer applications such as Microsoft Office, with willingness and ability to update and increase skills and knowledge as needed. Skill to set up and maintain computerized databases of plasmid and cell line inventories.

• High level mathematical computation skills required to maintain accurate data and to prepare reagent solutions.

SPECIAL CONDITIONS

• Willing and able to work safely with lab chemicals, bacteria, yeast and human cell lines.

• Must be willing to work overtime during the evening or weekend when necessary for experiments.

• Background check is required.