Research Associate - 113366
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#113366 Research AssociateExtended Deadline: Thu 1/20/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 12/08/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 12/17/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This position will be hired at the Staff Research Associate 2 or Staff Research Associate 3 level depending on the experience of the selected candidate.
The Moores Cancer Center is one of just 50 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Asimakopoulos lab focuses on multiple myeloma, the second most common blood cancer. Our lab has a longstanding track record in creating animal models for blood cancers. The second focus of our lab is tumor immunology, in particular how tumor matrix influences the interplay between immune system and cancer. For more information, see: www.asimakopouloslab.org.
Under supervision, the Research Associate will perform cell culture and propagation of tumor cell lines as well as transfection, lentiviral transduction, and cellular fractionation experiments. Responsible for maintaining and inventory of frozen cell stocks and routine testing for mycoplasma contamination. Process tumor specimens by extracting DNA and RNA, as well as perform cloning and subcloning of DNA fragments. Perform molecular biology/cell biologic assays, to study mouse models of cancer. With general supervision, handle mice for surgical procedures, as well as for tumor harvest and processing.
This position will serve as the point of contact with UC San Diego Animal Care Program. These duties will include submission of protocols for review, organization of approved protocols along with mandated record keeping. Train new lab members on ACP rules and regulations and ensure that their training is up to date. Manage a mouse colony of approximately 150 cages, perform weaning and genotyping duties. Act as the Area Safety Coordinator for assigned laboratory space. Responsible for EH&S compliance of the laboratory and the required training of lab personnel. Prepare biosafety protocols for submission to EH&S committees for review. Assist Principle Investigator with departmental budgetary and personnel matters. Integrate information obtained from scientific journals for use in the laboratory and disseminate this information to other lab members.
Responsibilities also include: preparing media and reagents, maintaining laboratory organization and laboratory supplies, purchasing laboratory supplies, and other critical functions as needed for the laboratory to run smoothly.
Theoretical knowledge of cell biology and molecular biology and/or Bachelor's degree in Biology, Chemistry, or other related areas, and/or equivalent education and experience.
Experience working with mice for surgical procedures, including anesthesia administration. Experience preparing reagents and cells for animal studies, working with animal models of tumors, and performing tumor harvesting. Experience managing a mouse colony, weaning, and genotyping.
Experience performing routine cell culture techniques, such as pipetting culture media onto growing human cells in culture plates and pipetting trypsin solution onto cell layers. Experience following basic research protocols and instructions closely.
Experience with extracting DNA and RNA from tumor specimens, and cloning and subcloning of pertinent DNA fragments. Experience performing PCR and qPCR reactions. Experience with transfection of plasmids, siRNA, or other genetic material (retroviruses, lentiviruses) into tumor cell lines.
Experience with Florescent Activated Cell Sorting (FACS) experiments and performing luciferase, clonogenic, anchorage independent growth, and other bio-assays.
Experience with preparing chemical solutions, including performing gel electrophoresis by mixing gel solutions and pouring into gel molds. Ability to perform standard mathematic computations (calculate molarity) accurately.
Knowledge of basic laboratory procedures and equipment, such as sterile techniques, preparation of buffers, centrifuges, balance pH meters and electrophoresis equipment.
Experience working with animal care protocols and laboratory safety coordination and compliance training. Ability to integrate information obtained from scientific journals for use in the laboratory and disseminate to other lab members.
Demonstrated ability in data management, analysis, interpretation, and description of scientific data in a publication format.
Basic experience with STATA and R (statistical software).
Experience and skill in using computers for basic word processing and data entry as well as PubMed and database searching and basic database analysis (e.g. BLAST). Computer skills and experience working with Word, Excel, and other commonly used programs.
Strong communication skills (both verbally and written) needed to interact professionally and effectively with a diverse population at all levels of organization in person, over the phone or in writing. Ability to use diplomacy, tact, and discretion in all interactions.
Employment is subject to a criminal background check.
Must be willing and able to work with small animals.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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