Research Assistant - 116516
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.
#116516 Research AssistantExtended Review Date: Mon 9/19/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 06/03/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 06/14/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center is one of just 50 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation - from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under supervision, assist with gastrointestinal stromal tumor (GIST) cell culture such as cell propagation, mycoplasma testing, cryopreservation, and compound treatment. Assist with protein lysate preparation and quantification, SDS PAGE, as well as immunoblotting. Assist with mouse genotyping using established PCR protocols. Work with mice and assist with procedures such as surgery, euthanization, injections, tissue and/or tumor harvesting, and therapeutic treatment. Assist with immunohistochemistry of frozen and fixed samples. Maintain lab organization, receive lab ordered supplies, and prepare common buffers/reagents/solutions for experiments. Assist in the preparation of data for publication.
Theoretical knowledge of molecular biology, cell biology, genetics, and/or biochemistry.
Familiarity with molecular biology techniques including PCR, RT-PCR, RNA/DNA isolation, and protein purification. Ability to perform SDS-PAGE.
Ability to perform aseptic cell/tissue culture techniques. Familiarity with cell culture techniques such as cell propagation, mycoplasma, contamination testing, and cryopreservation.
Ability to assist with immunohistochemistry of frozen and fixed samples.
Ability and willingness to handle and work with small animals. Experience to assist with mouse genotyping. Ability to assist in performing mouse surgeries, experiments, injections, euthanasia and tissue recovery. Experience with murine gastrointestinal models.
Familiarity with basic laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment.
Basic understanding of computer applications such as Word, Excel and PowerPoint.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Detail-oriented. Ability to work with little supervision. Ability to follow verbal and/or written instructions. Ability to follow-up on previously begun procedures. Ability to accurately record data and enter into appropriate medium.
Ability to adhere to established UCSD guidelines for safety and environmental issues. Familiar in proper handling of chemical and biohazard materials. Familiarity with sterile techniques.
Theoretical and practical knowledge of cellular and molecular biology; their techniques and approaches, with demonstrated ability to perform independent scientific inquiry or equivalent experience.
- Experience with immunoblotting for ER stress pathways.
- Experience with gastrointestinal stromal tumor cells.
- Internet search and software analysis capabilities. Knowledge and experience with GraphPad Prism.
- Employment is subject to a criminal background check.
- Must be willing and able to work with hazardous materials (biological and chemical).
- Must be willing and able to work with small animals.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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