111557 - Stem Cell Genomics Research Technician
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#111557 Stem Cell Genomics Research TechnicianFiling Deadline: Wed 10/6/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
UCSD Layoff from Career Appointment: Apply by 09/13/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/23/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Depending on experience of the selected candidate, this position will be hired at the SRA 1 or SRA 2 level.
The Stem Cell Genomics Core (SCGC) Facility is a shared service, multi-investigator laboratory staffed by one Staff Research Associate and one Core Manager. It is used by an increasing number of researchers who pays recharge costs to the facility. The Genomics Core facility hosts activities centered on improved sample preparation and secondary validation of sequencing results and is a cornerstone of UCSD Stem Cell Genomics. It provides services that do not overlap the services of the BioGem Core, but instead help maximize the usefulness of BioGem and other cores by developing technical solutions to processing bottlenecks. It fosters and promotes Genomics research at UCSD and partner institutes of the Sanford Consortium for Regenerative Medicine. The facility promotes Genomics research and the use of genomics data in a wide range of basic science and applied fields by developing methods that enable biological and cell scientists to harness the power of genomics data. The facility sustains ongoing research projects and allows the proposal of new projects necessitating such facilities. The facility provides services at the new Sanford Consortium for Regenerative Medicine building on North Torrey Pines.
The Staff Research Associate (SRA) assists the SCGC Manager in the execution of protocols on specialized equipment for genomics, single cell analysis, sequence processing, data management, secondary validation, and improvement of throughput for stem cell and research. The SRA carries out protocols for single-cell analysis, high throughput aPCR, MiSeq sequencing, and other general wet-lab equipment.
The SRA assists with library preparation for next generation sequencing, operates and troubleshoots mid throughput sequencers. The SRA assists with the organization and storage of DNA/RNA samples and corresponding data sets. The SRA assists with troubleshooting nucleic acid quality issues for qPCR and sequencing experiments, maintains cleanliness/safety of the lab, and performs routine maintenance on instruments. The SRA supports the orientation of Core users and the scheduling of experiments. The SRA performs primary data analysis and has functional knowledge of basic programming to assist in the transfer of large data sets to servers and data analysis pipelines.
The SRA works under close supervision and direction of the SCGC Manager to facilitate and encourage usage of the Stem Cell Genomics Core. The SRA applies working knowledge of genomics and cell biology to experimental design and data analysis.
Theoretical knowledge in biology, molecular biology, genetics, biochemistry or related field.
College level coursework in molecular biology, genetics and biochemistry, including relevant laboratory methods.
Excellent interpersonal and oral communication skills. Demonstrated skill to effectively and clearly communicate with tact, diplomacy, discretion, and flexibility to faculty, academicians, students, and staff including those of diverse ethnicity, culture, and foreign nationals. Ability to present information clearly and concisely.
Ability to use personal computers and a wide variety of software packages for word-processing, spreadsheets, database processing, presentations, web and publication design, and calendaring (e.g. Microsoft Office suite, Adobe suite, etc.). Knowledge of e-mail systems as a primary form of communication; accomplished skill at navigating the Internet for information retrieval.
Ability to learn new protocols for sequencing and single cell analysis, and ability to learn new software environments quickly and effectively.
Knowledge of sterile technique.
Bachelor’s degree in Biology or related field.
Familiarity with PCR amplification (theory and practice).
Ability to use next-gen sequencing platforms and preparing DNA/RNA libraries (Illumina).
Ability to carry out genomic research techniques including ability to keep clear and accurate records and skill to update and maintain databases.
Knowledge of MSDS’s (Material Safety Data Sheets), and the hazard communication and Injury and Illnesses Prevention Program. Knowledge of laboratory safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items such as gloves and goggles.
Lab experience and experience orienting others.
Employment is subject to a criminal background check.
Occasional evenings and weekends may be required.
Must be willing to work near labs where biological, chemical, and radiation hazards are present and animals and human embryonic stem cells are used in research.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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