111077 - Molecular Biology Research Associate
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#111077 Molecular Biology Research AssociateExtended Deadline: Mon 10/18/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/10/flu-vaccination-requirement-for-uc-students,-faculty-and-staff-issued-for-2021-2022.html
UCSD Layoff from Career Appointment: Apply by 08/18/2021 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 08/30/2021. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This posting will remain open until filled.
The Department of Medicine (DOM) is the largest department within the UCSD School of Medicine and is responsible for fulfilling the teaching, research and clinical missions of the University of California, San Diego School of Medicine. The department employs approximately 500 salaried faculty, 168 academic appointees, 331 residents, fellows and postdocs and 480 staff personnel.
The Research Assistant will design and implement research protocols and conduct experiments for ongoing research projects of the laboratory. Responsibilities include conducting molecular biological experiments (i.e., cloning, development and application of Adenoviral- and Adeno-associated viral vectors, Western Blot, immunoprecipitation, polymerase chain reaction (PCR), quantitative polymerase chain reaction (qPCR), single-cell RT-PCR), isolation of vascular endothelial/smooth muscle cells from mouse heart and lung, electrophysiological experiments (i.e., measurement of Ca2+ and ROS concentration in cytoplasm and mitochondria, assessment of GAP junction intercellular communication), and immunofluorescence experiment. The incumbent will perform data acquisition and analysis, grant and manuscript preparation, and presentation of findings at weekly lab meetings. Additional responsibilities include maintaining transgenic mice colony including breeding and genotyping, management of lab research records including methodological approaches, experimental records, and protocols, and maintaining a clean lab and the function of lab equipment. Aseptic techniques for tissue culture and animal handling are mandatory skills in our laboratory.
Proven ability and experience to isolate heart and lung vascular endothelial and smooth muscle cells and prepare primary cultured cells for molecular biological and cell physiological experiments.
Demonstrated ability and experience in gene cloning and silencing, production of Adeno- and Adeno-associated virus to transduce genes in the cells and tissues.
Proven ability and experience in Western Blot, immunoprecipitation, site-directed mutagenesis, PCR, qPCR, and single-cell RT-PCR.
Ability and experience in electrophysiological experiments and immunofluorescence experiments using a fluorescence microscope.
Proven skill to work with animals, as well as animal and human tissues.
Proficient knowledge of the following specialized programs; SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.
Ability to read scientific literature, understand appropriate study design, follow study procedures and protocol, and maintain participant confidentiality.
Theoretical and working knowledge in advanced molecular biology and cell physiology.
Aseptic techniques for cell culture.
Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.
Experience assisting with writing scientific manuscripts and contributing to teamwork in molecular biological experiments.
Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.
Effective and diplomatic oral and written communication and interpersonal skills. Ability to work with a variety of personnel from different laboratories and instruct in optimal procedures for assays.
- Employment is subject to a criminal background check.
- Must be available to work some weekends & evenings to complete experiments.
- Must be able to work with laboratory animals (mice).
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
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