Stem Cell Research Technician - 123324
Job Description
#123324 Stem Cell Research Technician
Filing Deadline: Thu 6/8/2023UC San Diego values equity, diversity, and inclusion. If you are interested in being part of our team, possess the needed licensure and certifications, and feel that you have most of the qualifications and/or transferable skills for a job opening, we strongly encourage you to apply.
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 5/29/2023 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 6/08/2023. Eligible Special Selection clients should contact their Disability Counselor for assistance.
DESCRIPTION
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined and are integral to the goals of the department.
The Department has undergone significant growth in recent years with a consolidated budget of approximately $106 million including sponsored projects expenditures of approximately $54 million and clinical revenue of over $ 52 million. This expansion of the Department’s research and clinical portfolio is expected to continue in the next few years.
The Department’s 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology, and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.
In 2001, physicians and leadership of the University of California, San Diego (UCSD), Rady Children's, and Children's Specialists of San Diego (CSSD) unified pediatric patient care, research, education, and community service programs, creating a university-affiliated children's health system to serve the region. Over the past two decades, an extensive list of joint programs has developed, with many physicians and researchers playing a role at both institutions.
This affiliation has consolidated the clinical, teaching, research, and public service programs of the UCSD School of Medicine's Department of Pediatrics with San Diego's only healthcare system dedicated to the health and well-being of children. In 2009, this partnership was furthered by the creation of Rady Children’s Specialists of San Diego, a Medical Foundation, and is now recognized as having global leaders in research, technology, translational medicine, education, and clinical excellence.
This position is for a technician who will aid in maintaining human-derived iPSC colonies, as well as the generation of NPCs and brain organoids. The position requires knowledge of standard molecular biology laboratory protocols (DNA/RNA extraction, PCR, immunohistochemistry, etc); and culturing of human pluripotent stem cells (passing, freezing, differentiating). The incumbent will assist PIs and other lab members with studies. Studies will include: 1) General growth, expansion, maintenance, of iPSCs; 2) Generation and characterization of NPCs, neurons, astrocytes and brain organoids; 3) Characterization of resultant phenotypes (by molecular, multi-electrode array, and imaging techniques); and 4) Molecular biology techniques involving the isolation of DNA to identify genotype and visualization of phenotypes in the brain. Procedures will include molecular biology (enzymatic extraction of DNA form tissue samples, PCR, gel electrophoresis), histology, light microscopy of sectioned mouse brain tissue as well as basic molecular and cellular biology techniques (immunohistochemistry of floating sections, Western blot, and maintenance of biochemistry equipment). Other tasks as required by the supervisor and project scientists.
MINIMUM QUALIFICATIONS
Theoretical and/or practical knowledge in human pluripotent stem cells and neural differentiation.
Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, and gel electrophoresis.
Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions.
Experience with the operation of a personal computer and software application, such as Access, MS Word, and Excel.
Ability to follow step-based experimental protocols.
Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.
Experience with general lab maintenance including cleaning, stocking supplies.
Ability to prioritize and perform efficiently.
Skill and ability to keep accurate data.
Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
Ability to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues.
Experience with maintaining and expanding rodent colonies.
PREFERRED QUALIFICATIONS
- Ability to read, comprehend, and discuss research materials, both in English and Portuguese.
SPECIAL CONDITIONS
Employment is subject to a criminal background check.
Willingness to work with standard hazardous laboratory compounds.
Willing to work evenings and weekends when necessary.
Pay Transparency Act
Annual Full Pay Range: $51,949 - $61,972 (will be prorated if the appointment percentage is less than 100%)
Hourly Equivalent: $24.88 - $29.68
Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for this position. The Annual Full Pay Range may be broader than what the University anticipates to pay for this position, based on internal equity, budget, and collective bargaining agreements (when applicable).
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.
To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.
UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.
UC San Diego Health maintains a marijuana and drug free environment. Employees may be subject to drug screening.
Application Instructions
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