Job Description

Payroll Title:
SRA 2 NEX
Department:
NEUROSCIENCES
Salary Range
Commensurate with Experience
Worksite:
La Jolla
Appointment Type:
Career
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
1
Work Schedule:
Days, 8 hrs/day, Mon-Fri

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#118788 Stem Cell Research Technician

Extended Deadline: Wed 10/5/2022

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy

UCSD Layoff from Career Appointment: Apply by 9/14/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 9/23/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.

DESCRIPTION

Perform standard laboratory procedures with Human Embryonic Stem Cells (hESC), neuronal precursor cells (NPCs), induced neurons (iNeurons), and mature neurons (mNeurons), and brain organoids. Under supervision, incumbent will work with PI and other lab members to refine protocols and to generate data from mutant cells for publication. Studies will include 1) General growth and maintenance of fibroblast and stem cell lines (adult and embryonic derived), 2) Gene manipulation of these stem cells with, CRISPR targeting, shRNA silencing or overexpression using transfection or lentivirus transduction, 3) Characterization of resultant phenotypes by molecular and imaging techniques, and 4) Molecular biology techniques to isolate RNA and quantify gene expression. Procedures will include molecular biology (generation of transgene constructs, PCR and RNA isolation) and microscopy as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, tissue culture and maintenance of biochemistry equipment). Independently perform tissue culture of primary cells, preparation of lentiviruses and viral infection techniques, maintain tissue culture facility stocks and inventory, perform regularly scheduled mycoplasma testing, ship and receive cell lines for collaborations. Perform cell culture electroporation in stem cells and independently perform routine biology and biology techniques including next generation sequencing techniques. Other tasks as required by the supervisor and project scientists. Incumbent will also be involved with general lab organization procedures.

MINIMUM QUALIFICATIONS

  • Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field as typically attained by a Bachelor's degree, or an equivalent combination of education and experience..

  • Demonstrated ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis, sterile technique.

  • Demonstrated experience in performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers, pH solutions, as well as various staining solutions.

  • Experience performing laboratory procedures with Human Embryonic Stem Cells (hESC) and, neuronal precursor cells (NPCs) and brain organoids.

  • Experience maintaining human embryonic stem cells, neuronal precursor cells, & hESC derived motor neurons in undifferentiated state.

  • Demonstrated experience generating lentiviral/retroviral culture medium for infection of hESCs, NPCs, & hESC derived motor neurons.

  • Experience working with DNA, RNA, protein in preparation for next generation sequencing or proteomics analysis.

  • Experience conducting experiments leading to differentiation patterns and the histological determination of specific phenotypes.

  • Experience with the operation of a personal computer and software such as Filemaker or other databases.

  • Proven ability to operate confocal, light, and fluorescence microscopes for documentation of results.

  • Demonstrated ability to follow and optimize complex and evolving step based cell culture protocols.

  • Demonstrated ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.

  • Experience with general lab maintenance including cleaning, stocking supplies.

  • Demonstrated ability to prioritize and perform efficiently.

  • Willing to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues.

  • Skill and ability to keep accurate data.

  • Demonstrated ability to read, comprehend, and discuss research materials.

  • Demonstrated ability to follow established Safety and Biohazard Material Guidelines.

PREFFERRED QUALIFICATIONS

  • Bachelor's degree preferred.

SPECIAL CONDITIONS

  • Willingness to work with animals, lentivirus, radioactive material, embryonic stem cells, human tissue and standard hazardous laboratory compounds.

  • Willing to work evenings and weekends to maintain workflow.


Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

Application Instructions

Please click on the link below to apply for this position. A new window will open and direct you to apply at our corporate careers page. We look forward to hearing from you!

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