Stem Cell Research Associate (SRA1) - 117179
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#117179 Stem Cell Research Associate (SRA1)Extended Deadline: Fri 8/19/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 06/28/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/08/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Depending on experience of the selected candidate, this position will be hired at the SRA 1 or SRA 2 NEX or SRA 3 level.
UCSD Department of Ophthalmology is one of the most innovative and productive academic departments in the country, offering challenging career opportunities in the diverse areas of cutting-edge research, and state-of-the-art clinical services. The department is committed to offering a dynamic learning environment and growing opportunities to its talented and dedicated employees.
The lab studies human eye development, disease and regeneration. This position seeks a highly motivated individual to carry out innovative research studying human eye development and disease using pluripotent stem cells to produce human retinal organoids and retinal monolayer cultures. This position will involve using CRISPR gene-editing to modify human stem cells for studies of retinal imaging and gene function and analytical skills including microscopy/image analysis and molecular analysis including qPCR and RNAseq to study changes in gene expression. Responsibilities will be to support all aspects of the lab including novel research including stem cell cultures, maintaining and differentiating stem cells, immunocytochemistry, PCR, support for molecular biology related to gene-editing of human pluripotent stem cells.
Incumbent will also be responsible for lab organization (e.g. inventory management), ordering of lab reagents, lab equipment maintenance and general support including to support preliminary data for grants grants, preparation of figures for manuscripts and other administrative support as needed. Applicants will also be in charge of relevant EH&S certifications for the lab. This is an excellent environment for learning new techniques and building upon existing skills. While this is a primarily a support role there is plenty of opportunity to publish new findings.
Theoretical knowledge of Molecular Biology, Cellular Biology, Biochemistry or Genetics, or equivalent fields and hands on lab experience at the bench.
Experience with cell culture, preferably stem cells and their differentiation.
Excellent interpersonal skills with strong, demonstrated ability to communicate orally and in writing. Possess skills of tact, poise, diplomacy, confidentiality and flexibility in a lab environment comprised of a diverse group of research personnel.
Experience performing some of the following techniques: PCR, cloning, CRISPR gene -editing, transfection of cells in tissue culture, molecular techniques to evaluate expression (qPCR, ddPCR) and function of targeted molecules.
Knowledge of basic lab safety requirements/procedures; EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials.
Experience with general lab maintenance including cleaning, stocking supplies, aliquoting.
Oral and written communication skills for presenting results to senior staff, writing reports, reviews and summaries.
- Must be able to maintain a detailed digital laboratory notebook accessible by the PI and other laboratory members.
- Experience with RNA sequencing is preferred but not required. If no experience, there must be interest in developing new bioinformatics skills related to RNAseq, single cell RNAseq as well as R- and python programming languages. Most of this can be taught on the job.
- Employment is subject to a criminal background check.
- Must be willing to work with pluripotent stem cells (including NIH approved lines of embryonic origin).
Must be available to work some weekends and evenings to complete experiments as needed.
* This job is for a minimum of two (2) years.*
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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