Stem Cell Research Associate - 118406
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#118406 Stem Cell Research AssociateFiling Deadline: Fri 9/30/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 08/26/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/07/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Effective 9/23: Depending on the experience of the selected candidate, this position will be filled at the SRA 2 or SRA 3 level.
The Department of Medicine is responsible for fulfilling the teaching, research, and clinical missions of the University of California, San Diego and employs 486+ full-time salaried faculty members and 100+ academic appointees. Staff personnel include 600+ staff in 9 bargaining units, students, and volunteers. There are 250 clinical faculty practicing at multiple sites and hospitals.
The overall focus of the laboratory is to understand how epithelial stem cells coordinate tissue renewal with the ultimate goal to harness stem cell regenerative potential to develop new strategies for precise tissue repair. Apply core techniques in cell biology, microscopy, mouse genetics and biophysics to address fundamental questions in stem cell biology during normal development and cancer progression. Under general supervision, the Senior Staff Scientist will independently conduct experiments, make observations, and keep detailed records of all aspects of experimental data related to research projects. Will independently perform molecular biological techniques in the development of plasmid and viral based gene transfer vectors and gene expression analyses (qRT-PCR and RNA-sequencing). Conduct procedures to carry out animal studies including breeding, injections, and maintaining and screening transgenic mouse colony. Perform in vivo fluorescent imaging and histology.
Incumbent will independently perform in vivo and in vitro assays, train new lab members, plan experiments, analyze data, and contribute to scientific papers. Incumbent will assist in data preparation and drafting of manuscripts assisting author or co-authoring scientific articles and some grant writing. Researching journal articles to find appropriate protocols and regulations for experiments. Prepare scientific abstracts and subsequent presentations for relevant scientific meetings as well as for progress reports. Adhere to laboratory environmental health and safety guidelines, including appropriate chemical waste and biological waste disposal.
Incumbent will be responsible for laboratory management including the ordering and maintenance of lab equipment and reagents, monitoring lab expenditures/grant spending, and overseeing the maintenance and organizing of laboratory stocks and ordering supplies, including management of laboratory sample inventories. Incumbent will serve as the laboratory safety officer to ensure that safe work practices are followed as dictated by EH&S guidelines.
Theoretical knowledge in cell biology, molecular biology genetics, stem cell biology and developmental biology or equivalent combination of experience and education.
Experience in designing, performing and analyzing research projects and understands what it takes to keep an organized yet fast-paced lab functional.
Experience and knowledge with animal procedures, breeding, handling, anesthesia, injection, transplantation, and colony maintenance as well as the ability to learn in utero-lentivirus injections and intravital fluorescent imaging.
Comfortable of handling and working with mice, primary human tissues and hazardous materials.
Expertise in molecular biology including RNA/DNA isolation, real-time PCR, viral production, plasmid design, cloning and preparation.
Experience with all aspects of tissue culture including freezing, thawing, counting cells and splitting cultures.
Demonstrated experience reading and using Material Safety Data Sheets. Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
Demonstrated experience working in and maintaining a sterile environment.
Experience with the use of general and special laboratory equipment such as ultracentrifuge, cryostat and microscopes.
Demonstrated skill in accurate record keeping and ability to organize, analyze, and calculate data. Knowledge in basic math for maintaining ledgers, income and expenditure reports.
Ability to keep accurate records of experimental procedures and results, to discuss them, and to prepare relevant oral and written presentations. Ability to present data in a clear and concise manner.
Strong computer skills and experience with the operation of all basic software applications such as Word, PowerPoint and Excel, and knowledge to work with a variety of statistical and analysis programs like Prism, and learn specialized imaging and analysis software (i.e Imaris).
Experience with word processing, database, spreadsheet, and graphics software with a willingness and ability to update and increase skills and knowledge as needed.
Ability to proactively and appropriately facilitate the maintenance of lab supplies and equipment. Ability to assist with general lab maintenance, including cleaning and restocking supplies and managing a regular schedule in the lab. Manual dexterity and legible handwriting.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Enthusiasm for learning new techniques and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.
Good organizational and time management skills.
Knowledge sufficient to coordinate studies with other departments and the lab. Skill to plan and organize work assignments in an efficient and productive manner.
Committed to work in a collaborative environment that values diversity, equity, creativity, and excellence.
Experience with flow cytometry and cell sorting.
Experience with tissue clearing and confocal microscopy.
Experience with high throughput sequencing (bulk, single cell and ATACseq) and working with R and/or Python.
Experience with lentivirus preparation and virus concentration.
Knowledge of UCSD Environmental Health and Safety requirements.
- Employment is subject to a criminal background check.
- Must be willing to work with mice and hazardous materials such as viruses and carcinogens.
- Willing to be flexible with work hours to include occasional evening or weekend work as required for animal care and experiments.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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