Stem Cell & Molecular Biology Research Associate - 116223
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#116223 Stem Cell & Molecular Biology Research AssociateFiling Deadline: Fri 5/20/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 5/10/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 5/20/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This position will remain open until a successful candidate has been identified.
This position will be hired at the Staff Research Associate 1 or Staff Research Associate 2 level depending on the experience of the selected candidate.
The Research Associate will assist with and perform standard laboratory procedures with Human Embryonic Stem Cells (hESC) and neuronal precursor cells (NPCs) and other experimental procedures. Incumbent will assist PI's and other lab members with studies. Studies will include 1) General growth and maintenance of stem cell lines, 2) Gene manipulation of these stem cells with silencing or overexpression constructs, 3) Characterization of resultant phenotypes (by molecular and imaging techniques), and 4) Molecular biology techniques to isolate RNA and quantify gene expression.
Procedures will include molecular biology (generation of transgene constructs, PCR and RNA isolation) and light microscopy of embryonic and neuronal cell lines as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, tissue culture and maintenance of biochemistry equipment). Perform tissue culture of primary cells, preparation of lentiviruses and viral infection techniques. Assist with cell culture electroporation in stem cells and perform routine biology and biology techniques. Other tasks as required by the supervisor and project scientists. Incumbent will also be involved with general lab organization procedures.
Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field.
Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis.
Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions.
Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel.
Ability to work with confocal, light, and fluorescence microscopes.
Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.
Experience with general lab maintenance including cleaning, stocking supplies.
Willing to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues.
Skill and ability to keep accurate data. Ability to read, comprehend, and discuss research materials.
- Employment is subject to a criminal background check.
- Willingness to work with animals, lentivirus, radioactive material, human tissue and standard hazardous laboratory compounds.
- Willing to work evenings and weekends when necessary.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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