DESCRIPTION

Incumbent will assist the PI and other senior researchers in research projects to determine the mechanisms of neurotransmitter switching. Studies will involve embryos of Xenopus laevis and adult mice. Techniques will include Western Blotting, in situ probes, embryo preparation and complex mating and breeding of mice. Under supervision, perform various molecular biological and biochemical techniques and participate in in vitro and in vivo experiments using techniques such as immunostaining and fluorescence microscopy.

Responsible for laboratory management, including oversight of the laboratory budget, the ordering of supplies, and the maintenance of a safe and secure workplace. Supervise care and breeding of lab animals. Hire, train and provide work direction to undergraduate student laboratory assistants. Perform other duties as assigned.

QUALIFICATIONS

• Demonstrated experience with molecular biological methods and procedures: DNA/RNA isolation/purification/ amplification, detection, agarose and acrylamide gel electrophoresis, western blotting

• Theoretical knowledge of cell biology, histology, molecular biology, immunology, general and organic chemistry, biochemistry, mammalian physiology, and genetics acquired through Bachelor's degree in biology, or an equivalent combination of education and work experience.

• Demonstrated analytical thinker, quick learner, and good listener with ability to follow oral and written instructions and protocols.

• Experience in the use of spectrophotometer, balance, vortex, osmometer, pH meter, autoclave, drying oven, micropipette and centrifuge

• Strong knowledge of embryological anatomy, ultrafine dissection, antibiotics, sterile techniques.

• Experience with weighing and measuring chemicals, preparing molar and normal solutions and serial dilutions.

• Skill in maintaining clear and concise records, skill in inventory of supplies and equipment.

• Excellent interpersonal communication skills including phone etiquette, and ability to supervise and interact with varied personalities

• Computer skills in entering, analyzing, and presenting data, using MS Word and Excel software with willingness and ability to update and increase skills and knowledge as needed.

• Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.

• Willingness and ability to work with frogs and mice. Humane handling of small animals. Knowledge of animal anatomy, care, feeding, watering & maintenance techniques. Ability to perform subcutaneous injections.

• Sensitivity to the needs of caged laboratory animals in compliance with AAALAC standards.

• Knowledge of regulations affecting use, care breeding and disposal of animals. Knowledge of UCSD animal protocols forms preferred.

• Ability to interview, train, and supervise assistants in the use of autoclaves, preparation of solutions, care of laboratory animals, use of catalogs and ordering supplies.

• Ability to work safely with hazardous materials. Experience and knowledge of proper handling, storage and disposal of chemicals, biochemicals, hazardous chemicals, and biohazardous materials including radioactive materials.

• Knowledge of California driving laws and possession of a valid driver's license.

SPECIAL CONDITIONS

• Willingness and ability to work occasional overtime and/or non-standard work hours as determined by the needs of experiments.

• Must be willing to work with small aquatic animals (frogs and tadpoles) and mice.

• Must be willing and able to work safely with hazardous materials.

• Must be able to occasionally lift, carry and/or push 20-50 lbs.

• Must have a driver’s license to be able to access the Greenhouse.

• Background check required.