DESCRIPTION

The mission of the UCSD Center for Epigenomics is to develop a quantitative and molecular framework to study the function and regulation of the human genome, with the ultimate goal to understand the molecular basis of cancer and other human diseases. Epigenomics is at the forefront of Precision Medicine. The epigenome, a term referring to the state of DNA methylation and the covalent modifications of histone proteins in a cell, plays a central role in regulating the functional output of the genome during development. Changes in the epigenome that result from exposure to stress, pathogenic conditions and during aging can determine the patterns of gene expression and predispose an individual to diseases. Epigenome research promises to significantly improve our understanding of the factors that drive human development and disease. The UCSD Center for Epigenomics works with researchers on the UCSD campus and beyond to foster the application of epigenomic concepts and technologies to clinical, translational, and basic research.

Under the general supervision of the Center for Epigenomics' Associate Director, the Senior Epigenomics Research Associate will independently engage in research projects by designing and performing experiments to map cells’ transcriptomes and epigenomes such as ATAC-seq, ChIP-seq, CUT&RUN, scATAC-seq, scRNA-seq, scMultiome, and Paired-Tag.

The incumbent will act as the primary driver for multiple collaborative research projects by implementing new workflows and standard operating procedures across novel and difficult sample types including tissues from atypical model organisms and precious clinical samples such as tissue biopsies. The incumbent will interpret the results to further optimize and scale these workflows. The incumbent will also play a key role in technology development by automating these workflows where appropriate on liquid handling robots to maximize throughput and minimize cost per sample.

The incumbent will independently design experiments and be responsible for careful record keeping, operating laboratory equipment including the Beckman Biomek i7 liquid handler, troubleshooting, quality control, and preparing complete written reports potentially resulting in publications. Additional duties may include contributing to laboratory management, organization of laboratory reagents, and maintenance of laboratory equipment. We seek a candidate who is enthusiastic about learning cutting-edge genomic techniques and working in a dynamic "start-up" atmosphere similar to what might be found in the biotechnology sector, yet within a vibrant academic research university.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of Molecular Biology and Biology Research, typically attained during a MS or PHD Degree or equivalent research experience.

• Experience, skill and knowledge of research design and methodology to achieve experimental goals.

• Extensive experiments generating high-quality epigenomics data from atypical, difficult, or precious samples such as minute tissue biopsies

• Demonstrated experience running epigenomic experiments using liquid handling robotics.

• Strong ability to perform complex molecular biology techniques such as PCR, DNA preparation/extraction, and gel electrophoresis.

• Experience with the operation of a personal computer and software application, such as MS Word and Excel. Experience in a UNIX command environment is a plus, but not required.

• Demonstrated ability to read interpret and discuss complex research materials.

• Proven ability to communicate effectively with clinical staff, research staff, and function as a member of a research team.

• Enthusiasm for learning new techniques, and working in a fast-paced "startup" atmosphere.

• Experience with advanced lab maintenance and proven ability to provide work direction and training to junior lab members.

• Strong communication skills (both verbal and written) to interact professionally and effectively in the work environment, as well as effectively and accurately document research procedures.

• Proven experience and skill in using computers. Knowledge of computers and use of scientific software for data analysis.

PREFERRED QUALIFICATIONS

• Hands-on experience with epigenomic assays such as ChIP-seq, RNA-seq, or ATAC-seq.
• Hands-on experience with high end laboratory equipment such as robotic platforms, cell sorters, or DNA sequencers.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.
• Occasional evenings and/or weekends may be required.
• Willingness to work with animals, lentivirus, radioactive material, human tissue and standard hazardous laboratory compounds.