Neurosciences Research Assistant - 113602
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.
#113602 Neurosciences Research AssistantFiling Deadline: Fri 2/4/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 12/22/2021 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 1/3/2021. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Center for Neural Repair in the UC San Diego Department of Neurosciences studies gene and cell therapies for spinal cord injury and for neurodegenerative disorders such as Alzheimer's disease.
Under supervision and independently, incumbent will provide surgical and veterinary support in a neurosciences research laboratory studying gene therapy in animal models of neurodegenerative disease. Primary responsibilities include rodent surgery, post-operative care, perfusion, and dissection, as well as maintaining transgenic mouse lines. Additional responsibilities may include histology (tissue sectioning, immunohistochemical staining, and microscopy), molecular biology (preparation and validation of plasmid DNA, restriction digest, gel electrophoresis, western blot, ELISA, and qPCR), and cell biology (subculture of immortalized cell lines, live imaging of cultured cells, and immunocytochemistry). Will maintain detailed records, perform rigorous data management, and collaborate effectively with a diverse laboratory staff.
Theoretical knowledge in biology or related field, as typically attained by a Bachelor’s degree and/or an equivalent combination of education and experience.
Ability and knowledge in handling and performing survival surgery in rodents.
Ability and knowledge performing rodent perfusion and dissection.
Experience with transgenic mouse breeding and genotyping.
General familiarity with tissue sectioning and immunohistochemistry.
General familiarity with cell and molecular biology techniques.
Strong computer skills and experience with word processing, spreadsheets, and graphics software. Ability to learn new software for analysis of microscopic images.
Excellent verbal, written, and electronic communication skills and ability to keep accurate records, prepare study reports, and write detailed protocols.
Strong interpersonal skills and the ability to collaborate effectively with a diverse laboratory staff, politely resolve issues, and communicate clearly.
Knowledge of basic lab safety requirements and procedures including EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials.
Solid scientific thinking skills and ability to independently troubleshoot assays, identify and correct problems, improve protocols, and train others.
- Experience performing stereotactic injection of rodent brain.
- Experience performing intrathecal infusion in rodents.
- Experience performing light, fluorescent, and confocal microscopy.
- Experience performing specific molecular biology techniques including: bacterial culture; plasmid DNA subcloning, verification, and purification; western blot; ELISA; PCR; qPCR; RT-qPCR.
- Experience performing specific cell biology techniques including: subculture of immortalized cell lines; electroporation and transfection of cultured cells; live imaging; immunocytochemistry.
- Employment is subject to a criminal background check.
- Must be willing and able to work with live rats and mice in a research related environment.
- Must be willing to work on occasional weekends and holidays.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.
UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.