Lab Manager and Stem Cell Research Associate - 118385
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.
#118385 Lab Manager and Stem Cell Research AssociateExtended Review Date: Fri 9/16/2022
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 08/23/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/01/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
UCSD Health Sciences is a $1 billion dollar plus organization encompassing 17 academic departments (15 clinical, 2 basic sciences), 2 preacademic/hospital departments, 2 professional schools (School of Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences), 2 hospitals, and various other programs and units all dedicated towards fulfilling the Health Sciences missions. The UCSD Department of Cellular and Molecular Medicine (CMM) is one of two basic science units in the School of Medicine where world-class researchers study fundamental cellular processes and pathways using a variety of techniques from classical genetics and fluorescence microscopy to high throughput genomics, systems biology, and crystallography. Our 37 faculty and over 200 postdoctoral scholars, graduate students, and research staff work on the cutting edge of biology, cutting across fields from basic biochemistry and genetics to genomics, systems biology and stem cell biology.
The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines and provide diversity to meet the interests of a broad spectrum of students and scholars. The diverse mix of ages, backgrounds, and talents creates a robust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically advanced, and deeply interested individuals who are ready to become a part of our dynamic research and educational environment.
The Institute for Network Medicine [www.inetmed.ucsd.edu] unites several research disciplines to develop disruptive solutions, transform life sciences, and technology and enhance the quality of human life. Founded in Mar of 2018, iNetMed houses three centers, which are collaboratively developing new, human-centered approaches to drug discovery and technological innovation. These positions are geared towards "human-centered drug discovery:
Human-Centered Drug Discovery: The world’s drug discovery machinery has a tragic paradox. Academic labs, pharmaceutical companies,6*460 and biotechs have better tools than ever before: supercomputers, genomic sequencers, PCR. Despite these incredible technologies, the drug discovery success rate is lower now than it was in the 1970s. There are two reasons for this: First, the era of ‘big data’ has not been translated into effective drugs, as was initially expected. Second, current approaches for target discovery rely far too much on inbred animal models (mice, rats, etc.), and drugs that work wonders in these animals. These pre-clinical studies fail far too often, then too late in clinical trials, making the process wasteful and imprecise. To overcome these challenges, iNetMed’s two Centers, the Center for Precision Computational System Network (PreCSN) and HUMANOID Center of Research Excellence (CoRE) are working collaboratively to provide an end-to-end new paradigm and platform for drug discovery that begins with target identification (at PreCSN) and ends with target validation in human disease models (at HUMANOID).
The Incumbent will mostly work within the UC San Diego HUMANOID CoRE (humanoid.ucsd.edu), an organoid-based research core that strives to build disease models for testing therapeutics.
Under supervision, research associate will independently perform standard laboratory procedures with adult human stem cells and primary cells, human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), as well as other experimental procedures. Perform a number of standard repetitive laboratory procedures with stem cells, primary cell lines and immortalized cell lines, as needed, with the intent to build disease models that will aid in drug discovery for grand challenges in human diseases that are still without cure.
Use basic molecular biology and microscopy techniques to independently conduct a number of experimental procedures. The research associate will use microscopes, high content imaging, plate-readers, flow cytometers, cell fractionarors, isolators, microfluidic chambers, and available robotics to assay effects of small molecules on stem cells and organoids grown from stem cells will be necessary. The incumbent will perform standard experimental protocols in tissue culture to meet the needs of the research projects, providing ideas and rationale to help improve tests that are unsatisfactory.
Lab managerial duties to include responsibility for laboratory management and supervision of all activities related to research logistics (e.g., stocking, equipment maintenance, inventory and ordering), research safety, compliance and training of the entire unit (HUMANOID), which will include at least one Staff Research Associate II or above and two Staff Research Associate I's, lab assistants and trainees (postdoc fellows, undergraduate and graduate students and visiting scholars). Duties will also include assistance in teaching activities, and training other staff/students following standardization of new techniques.
With minimal supervision, the incumbent will edit and maintain database of human tissue and organoids in the HUMANOID biorepository, maintain up-to-date clinic IRBs (Consent forms and IRB protocols), and serve as runner if needed to secure patient-derived tissues from clinics and bring them to the HUMANOID for organoid isolation. To maintain compliance with IRB protocols, the incumbent will monitor that proper IRB protocols are used for submitting downstream human samples to the histology Cores on campus and similar facilities for other experiments. For these tasks, the incumbent will need to use Word, Excel, and PowerPoint software programs and computers. Must be willing to drive/access public transportation as needed.
Theoretical knowledge of molecular and cellular biology or closely related field as typically acquired at a collegiate level or equivalent combination of education and experience.
Demonstrated experience performing DNA techniques.
Lab experience, knowledge and understanding of sterile techniques and mammalian cell culture practices. Knowledge and experience with numerous types of laboratory equipment related to uses, maintenance, and repair. Ability to learn new laboratory procedures quickly.
Thorough knowledge of small molecule library screening and robotics.
Proven experience working with various computer software, hardware, standard office equipment and lab equipment.
Acute attention to detail, resourceful, capable of handling the changing needs of the research lab.
Proven knowledge of or experience in bacteriology and bacteria cell competency.
Strong ability to use mathematical skills to analyze and develop statistical data.
Proven experience in sterile cell culture techniques for culture of Human Embryonic Stem Cells and other mammalian cells.
Thorough knowledge of or proven experience performing repetitive and specialized laboratory experimental procedures. Experience following experimental protocols.
Experience evaluating the effect of small molecules on the organoids (drug discovery) and monitor cell health via MTT and LDH assays
Proven ability to perform experiments with adult and hPSCs for immunofluorescent labeling, fluorescence (confocal) or electron (TEM) microscopy, other plate-based assays, and for small molecule library screens.
Demonstrated experience performing basic HTP assay set ups with organoids
Experience conducting immunohistochemistry, imaging, and quantification of FFPE slides using either scoring algorithms or software programs, as needed.
Proven ability to contribute to modeling new organs via organoid models and their linked disease models.
- Job offer is contingent upon satisfactory clearance based on Background Check results.
- Occasional evenings and weekends may be required.
- Work in environment where biological (including infectious agents and blood bourne pathogens) and chemical hazards are present. Research on the human adult and embryonic stem cell and Lentiviral cultures are used.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.
To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.
UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.