Lab Assistant 2 - 121829
#121829 Lab Assistant 2Filing Deadline: Thu 3/30/2023
UC San Diego values equity, diversity, and inclusion. If you are interested in being part of our team, possess the needed licensure and certifications, and feel that you have most of the qualifications and/or transferable skills for a job opening, we strongly encourage you to apply.
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
UCSD Layoff from Career Appointment: Apply by 03/20/2023 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 03/30/2023. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Department of Pathology at UC San Diego aims to achieve the highest standards in patient care, research and education. The Department provides diagnostic pathology services in a wide variety of subspecialties with highly trained subspecialists who are recognized for their expertise. The Department’s research programs are diverse and interface with a wealth of basic and translational research programs that are a hallmark of UC San Diego.
This Lab Assistant will work in a laboratory investigating the role of the microbiota in modulating immune responses during health and disease. Utilizing the combination of genetically engineered mice maintained under germ-free or defined microbial communities, our lab is able to explore the multifactorial contributions to disease pathogenesis. Under supervision, the applicant will assist in performing experiments in immunology and microbiology to characterize mucosal immune responses to gut microbes using mouse models of disease. These experiments include monitoring and treatment of laboratory animals, tissue collection, histological assessment, and downstream immunological/microbiological assays.
Other responsibilities include tissue culture, cell isolation and other cell-based assays, basic cell and molecular biology techniques, and data analysis. Experience with computational analyses is desired. Additionally, this applicant will assist in performing laboratory maintenance and administrative duties including ensuring compliance with IACUC and EH&S requirements, ordering and tracking of supplies, data organization, teaching basic laboratory techniques and preparing data for presentation.
Graduation from high school or a GED and two (2) years of laboratory experience or two (2) years of college including courses in the natural, physical or social sciences and one (1) year of laboratory experience, or an equivalent combination of education and experience; and knowledges and abilities essential to the successful performance of the duties assigned to the position.
Theoretical knowledge in immunology or a related biomedical science.
Experience and demonstrated ability to initiate and maintain mouse experiments including treatment and observation for disease. Experience with mouse handling, breeding, genotyping, anesthesia, surgeries, injections/treatments, and tissue harvest.
Experience and ability anaerobic culturing studies and bacterial isolation, including growth curves in nutrient-rich and defined media, and associated analyses
Experience and demonstrated ability in extracting DNA/RNA, PCR, PT-PCR, qPCR, analysis and troubleshooting.
Experience and ability to initiate and maintain animal experiments multiple different eukaryotic cell lines including freezing, thawing, storage, and plating cells in preparation for experiments. Experience with sterile techniques, trouble shooting of contamination problems, and care of incubators and liquid nitrogen freezers.
Experience in protein chemistry/purification, gel electrophoresis and transfers, various gel and membrane stains, ELISAs, western blots, and densitometry and analysis on resulting data.
Knowledge of microbiological techniques, including culturing of aerobic/anaerobic bacteria, aseptic technique, and generating glycerol stocks with documentation.
Ability to follow written and verbal protocols, techniques, and instructions.
Excellent communication and interpersonal skills over a wide range of situations and individuals needed to interact professionally and effectively in the work environment in person, on the phone, and in writing to a diverse population; including persons from foreign countries, the business community, academicians, students and staff with a high degree of diplomacy, mature judgment, tact, and flexibility.
Knowledge of proper formats, grammar, and spelling in English and proofreading ability sufficient to compose, edit, and publish professional correspondence as well as effectively and accurately document research procedures. Ability to read, comprehend, discuss, and present research materials.
Knowledge of bio, chemical, and radiation safety techniques and practices. Demonstrated ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Strong organizational skills to order and maintain lab reagents and supplies, and keep accurate lab records and computer files. Experience in ordering, maintaining, trouble-shooting, and calibrating general laboratory equipment (e.g. centrifuges, incubators, microscopes, water baths, etc.).
Experience with the operation of a personal computer and software applications, such as Microsoft Office (Word, Excel, Access), Photoshop, Acrobat.
Experience with reading and interpreting primary literature to obtain background knowledge in new research area and to develop new protocols or in troubleshooting assays.
- Experience with UCSD ordering, EH&S, and facilities policies and business systems.
- Employment is subject to a criminal background check.
- Must be willing to work with lab animals.
- Must be willing to work in gnotobiotic isolators/glove boxes.
- Must be willing to work with hazardous materials.
- Must be willing to work occasional evenings and weekend days, as required.
Pay Transparency Act
Annual Full Pay Range: $43,159 - $50,738 (will be prorated if the appointment percentage is less than 100%)
Hourly Equivalent: $20.67 - $24.30
Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for this position. The Annual Full Pay Range may be broader than what the University anticipates to pay for this position, based on internal equity, budget, and collective bargaining agreements (when applicable).
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.
To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.
UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.
UC San Diego Health maintains a marijuana and drug free environment. Employees may be subject to drug screening.
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Job Reference #: 121829