Genomics and Laboratory Research Associate - 110028
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#110028 Genomics and Laboratory Research AssociateExtended Review Date: Tue 1/18/2022
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This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 07/07/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/15/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center is one of just 51 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs. The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation - from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
The Genomics and Laboratory Research Associate will be expected to contribute to the independent design, conduct, and analysis of experiments and their results and also preparation, presentation, and publication of results under the direction of the Principal Investigator (PI). Make significant innovative contributions pertaining to the design and overall direction of scientific research projects and propose possible changes or new directions in the course of the investigations. Read and evaluate scientific articles relevant to current research studies, finding new sources of information. The incumbent will be responsible for applying or adapting scientific techniques and assisting with the planning and scheduling of research procedures, recording the results of studies, compiling and analyzing data including statistical analyses, and preparing charts, graphs and presentations.
Scientific intellect especially as it pertains to cancer molecular biology and computational genomics is essential. Experience with bioinformatic tools and programming experience is also essential for analysis of high throughput genomic sequencing data. Work will include sequencing alignment, analysis, comparative statistics and algorithm development. Responsibilities will also include cell culture, growth and migration assays, isolation of nucleic acids and proteins, and quantitative analysis with PCR. Lastly immunologic correlates including EliSPOT assays and T-cell reactivity assays will be evaluated in relationship to genomic alterations. The incumbent will be working with murine animal models, maintain animal colony, maintain laboratory equipment, order supplies as needed and assist laboratory management to provide support for students, postdocs and general laboratory operations.
Our research work seeks to understand post transcriptional changes, including alternative splicing events, which are prevalent in head and neck cancer. We utilize computational biology methods to achieve a deeper understanding of these post transcriptional genomic alterations that are not readily apparent through sequencing alone. Splicing alteration and post transcriptional alterations represent an understudied gap in genomic knowledge that seeks to understand changes that occur between DNA mutations and ultimately expressed protein phenotypes in tumors. By harnessing computational tools, we can come closer to translating this genomic knowledge towards the delivery of precision cancer care in head and neck cancer. Other duties assigned as needed.
Advanced theoretical knowledge and/or Bachelor's Degree in molecular biology, and/or related work experience.
Proven experience with bioinformatic analysis including proficiency in use of R.
Demonstrated experience performing RNA and protein isolation.
Experience performing sterile eukaryotic cell culture techniques, PCR and agarose gel electrophoresis.
Experience handling and working with small animals, including demonstrated experience conducting mouse surgeries, experiments, and injections. Knowledge in conducting euthanization of small animals.
Strong experience and knowledge in coordinating major mouse experiments, such as planning injection and drug treatment dosing schedule.
Experience with protein biochemistry techniques; including quantification, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations and gel electrophoresis.
Strong communication skills (verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Proven experience using laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment.
Experience in proper handling of chemical and biohazard materials. Experience with sterile techniques. Experience to adhere to established UCSD guidelines for safety and environmental issues.
Experience with computer software such as Excel and R to analyze data, store results and compute raw data. Experience of computer applications such as Word, Excel and PowerPoint. Internet search and software analysis capabilities.
Experience in troubleshooting problems for quality control on every immunochemical and histochemical procedures.
- Experience in cellular immunology techniques, such ELISPOT, cytokine detection.
- Experience using histological and immunohistochemical techniques to evaluate protein localization in fixed cells or tissues.
- Experience performing light, fluorescence and confocal microscopy techniques to visualize protein expression in tissue sections.
- Employment is subject to a criminal background check.
- Must be willing and able to work with hazardous materials (biological and chemical).
- Must be willing and able to work with small animals.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!
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