112580 - Staff Research Assistant
#112580 Staff Research AssistantExtended Deadline: Mon 11/29/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy
Job posting will remain open until position is filled.
This is a 100% limited position for one (1) year with the possibility to extend or conversion to career status depending on funding and project needs.
Under the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of endomembrane organization by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells.
Research techniques involve molecular cloning strategies, tissue culture maintenance and transfection, expression and purification of recombinant proteins, model membrane systems, as well as super-resolution microscopy, image analysis and data management. Incumbent will provide general lab support including ordering supplies, maintaining common lab equipment, and ensuring lab is clean, safe, and in compliance with regulations.
Theoretical and practical background and knowledge in Molecular, Cell Biology and Biochemistry acquired through a BS degree or higher in Biology or related field OR an equivalent combination of education and laboratory experience.
Experience with basic and general techniques in molecular biology, including PCR, gel electrophoresis, DNA cloning, and plasmid preps.
Experience in affinity purification of recombinant proteins and protein analysis (SDS-PAGE, Western blot)
Knowledge of scientific terminology related to cell, molecular biology and/or biochemistry.
Knowledge and skill with sterile technique for bacterial culture. Familiarity with basic tissue culture techniques
Experience with phase contrast and fluorescence microscopy.
Experience with protein purification from mammalian cell culture
Excellent (English) verbal, written, and electronic communication skills that facilitate professional, effective, and courteous interactions with diverse personalities. Skill to work as part of a research team.
Experience with and understanding of basic laboratory equipment (centrifuges, light microscopes, balances, pH meter, incubators, spectrophotometers, and electrophoresis apparatus)
Skill and knowledge to maintain stocks of essential supplies and reagents, to purchase lab supplies, and to ensure lab equipment is running efficiently.
Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Familiarity with Apple and PC computers and ability to work independently with a variety of computer applications such as Microsoft Office, with willingness and ability to update and increase skills and knowledge as needed. Skill to set up and maintain computerized databases of plasmid and cell line inventories.
Basic mathematical computation skills required to maintain accurate data and to prepare reagent solutions.
Knowledge of UC San Diego and Division of Biological Sciences regulations, preferred
Experience with model membrane systems (liposomes, supported bilayers), preferred
Experience in analyzing microscopy data using ImageJ or similar tool.
Willing and able to work safely with lab chemicals, bacteria, and human cell lines.
Must be willing to work overtime during the evening or weekend when necessary for experiments.
Background check required.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.