Job Description

Payroll Title:
SRA 3
Department:
NEUROSCIENCES
Salary Range
Commensurate with Experience
Worksite:
Campus
Appointment Type:
Career
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
2
Work Schedule:
Days, 8 hrs/day, Monday-Friday

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#112547 Electron & Light Microscopy Specialist

Filing Deadline: Thu 12/2/2021

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy

UCSD Layoff from Career Appointment: Apply by 10/22/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 11/2/22. Eligible Special Selection clients should contact their Disability Counselor for assistance.

This position will remain open until a candidate has been successfully identified.

DESCRIPTION

Provides hands-on technical support in electron and light microscopy methods. Collaborates with researchers from several laboratories, aids the research group and independently conducts laboratory investigations at NCMIR.

Performs and develops both specimen preparation and sectioning techniques in the areas of high resolution transmission and intermediate high voltage electron microscopy, immunofluorescence, immunocytochemistry, protein labeling, serial sectioning, computer-aided 3-dimensional reconstruction, tomographic reconstruction within thick biological specimen.Develops, modifies and implements freeze fracture techniques, 2-photon imaging and confocal microscopy.

Obtains large scale brain mapping data and examines and acquires high quality images of specimens for both data analysis and publication.Teaches and trains undergraduate students, graduate students, post-docs and collaborators on equipment and laboratory procedures used in confocal and electron microscopy.

MINIMUM QUALIFICATIONS

  • Theoretical knowledge of histology, histochemistry, molecular and cellular biology as well as chemistry and biochemistry.

  • Skill in providing individual instruction on the use of the transmission electron microscope, confocal microscope, and peripheral support of these instruments.

  • Familiarity and skill with software for processing and creating two-dimensional and three-dimensional images, especially for tomography.

  • Ability to understand and evaluate scientific literature. This includes all aspects of the research project and to interpret results for scientific publications.

  • Excellent written and oral communication skills and ability to juggle multiple projects at once with good organization skills.

  • Experience in the production of high quality vibratome sections for confocal microscopy. Familiarity with neuroanatomical landmarks and cellular components in brain slices.

  • Experience in the production of high quality light images and both electron microscopy, CCD images, and electron micrographs.

  • Experience and skill with transmitted light microscopy, laser scanning confocal, transmission electron microscopy, and high voltage electron microscopy. Ability to increase skill with multi-photon microscopy.

  • Experience to produce high quality thin and semi-thin sections for electron microscopy with glass and diamond knife. Including serial sectioning of thick sections for ultra-high electron microscopy.

  • Understanding of theory and practice of chemical tissue fixation, high pressure freeze fixation and freeze fracture for localization procedures and ultrastructural procedures used in transmission electron microscopy, confocal microscopy, and light microscopy.

  • Understanding both the theory and practice of various cellular localization labeling techniques for light, confocal, and transmission electron microscopy. Experience with certain cellular localization procedures. Ability to learn new labeling techniques.

  • Experience with high resolution localization labeling techniques (such as colloidal gold etc.) for electron microscopy. Ability to learn new labeling techniques.

PREFERRED QUALIFICATIONS

  • Knowledge of graphing software (e.g., GraphPad prism) and basic understanding of statistical analyses is a plus.
  • Basic familiarity with Linux operating system.

SPECIAL CONDITIONS

  • Employment is subject to a criminal background check.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

Application Instructions

Please click on the link below to apply for this position. A new window will open and direct you to apply at our corporate careers page. We look forward to hearing from you!

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