Job Description

Payroll Title:
SRA 1
Department:
PEDIATRICS/Genetics
Salary Range
Commensurate with Experience
Worksite:
Campus
Appointment Type:
Career
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
1
Work Schedule:
Days, 8 hrs/day, Monday-Friday

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#112484 Stem Cell Research Technician

Filing Deadline: Tue 11/30/2021

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus and influenza before they will be allowed on campus or in a facility or office. For more information visit: Flu Vaccine Mandate / COVID Vaccine Policy

UCSD Layoff from Career Appointment: Apply by 10/20/2021 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 10/29/2021. Eligible Special Selection clients should contact their Disability Counselor for assistance.

Depending on the experience of the selected candidate, this position will be hired at the SRA 1 or SRA 2 NEX level.

DESCRIPTION

The incumbent will assist with and perform standard laboratory procedures with Human Pluripotent Stem Cells (hESC/iPSC) and develop brain organoids as well as other experimental procedures. Incumbent will assist PIs and other lab members with studies. Studies will include: 1) General growth, expansion, maintenance, population enrichment, and induction of specific neural lines from stem cells. Induction of neural lines will involve the generation of: human astrocytes (using 3D neurosphere culture techniques), organoids, and human neurons. 2) Gene manipulation of stem cells with silencing or overexpression viral vectors constructs. 3) Characterization of resultant phenotypes (by molecular, multi-electrode array, and imaging techniques). 4) Molecular biology techniques involving the isolation of RNA and protein to quantify gene and protein expression.

Procedures will include molecular biology (generation of transgene constructs, PCR, RNA isolation and quantification), histology, light microscopy of embryonic and neuronal cell lines as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, immunohistochemistry, Western blot, ELISA, tissue culture and maintenance of biochemistry equipment). Perform routine tissue culture of primary cells, preparation of lentiviruses and viral infection techniques. The candidate will be responsible for oral and written reports every other week.

MINIMUM QUALIFICATIONS

  • Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field.

  • Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis.

  • Capable of performing biochemistry and laboratory techniques and experience to prepare buffers and solutions, as well as various staining solutions.

  • Experience with general lab maintenance including cleaning, stocking supplies.

  • Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel.

  • Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.

  • Ability to prioritize and perform efficiently. Skill and ability to keep accurate data.

  • Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.

PREFERRED QUALIFICATIONS

  • Experience with UCSD policies and procedures and basic safety rules and issues.
  • Experience with human pluripotent stem cells.

SPECIAL CONDITIONS

  • Employment is subject to a criminal background check.
  • Willingness to work with animals, viral vectors, radioactive material, human primary tissue and standard hazardous laboratory compounds.
  • Willing to work evenings and weekends when necessary.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

Application Instructions

Please click on the link below to apply for this position. A new window will open and direct you to apply at our corporate careers page. We look forward to hearing from you!

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