112311 - Research Associate (SRA 3)
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#112311 Research Associate (SRA 3)Filing Deadline: Tue 10/26/2021
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The Moores Cancer Center is one of just 49 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation - from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under minimal supervision, independently performs research duties in an Oncology/Urology research lab, specializing in testicular cancer research. Perform experiments, make observations, and record experimental data related to research projects. Use molecular biology to develop plasmid and viral based gene transfer vectors. Perform cell-based assays for tumor targeting treatments based on in depth review of the literature and knowledge of nuclear hormone-based and signal transduction pathway targeting therapeutics and chemistry. In addition, responsible for composing and revising publications; presenting research findings at academic conferences; and writing and revising funding/grant proposals.
Perform animal studies including breeding transgenic mice, injections, and maintaining and screening transgenic mouse colony. Process in vivo fluorescent and bioluminescent imaging, necropsy, tissue trimming and histology. Prepare tissue cultures of primary lymphocytes and propagation of tumor cell lines. Extract RNA, reverse transcribe cDNA, perform mini preps of plasmids and their analysis by gel. Perform basic cellular immunology tests to assess immunity such as FACS and CFSE proliferative assays, and automatic cells counting. Media and buffer preparation. Maintain lab records and inventory/order supplies.
This posting has been slightly modified.
Advanced theoretical knowledge of microbiology, immunology, cellular, molecular and/or testicular cancer biology or a related field as typically attained by a Bachelor's degree, or an equivalent combination of education and experience.
Experience with caring for and handling small lab animals (mice) including administering medications and anesthesia to perform phlebotomy and minor surgical procedures on animals. Experience euthanizing small laboratory animals.
Experience working with human tissues; tissue trimming, histology analysis, and knowledge in necropsy. Knowledge of current testicular cancer disease models and understanding of clinical features of testicular cancer and treatments.
Experience with establishing and running in vivo pre-clinical models including experience with animal work, to independently perform intra-venous, intra-cardiac and intra-femoral injections in mice, oral gavage and drug treatments of our mouse models in vivo. Perform tumor measurements with calipers, IVIS.
Experience preparing media for tissue culture including establishing and maintaining tissue culture, preparing complex reagents and media, processing patient blood samples. Experience with tissue culture including freezing, thawing, counting cells and splitting cultures.
Demonstrated experience and knowledge of flow cytometry.
Demonstrated experience applying DNA/RNA purification in order to devise primers for successful application to meet protocol requirements and obtain results.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials. Excellent interpersonal skills in order to interact and communicate with nurses in the patient clinic.
Experience conducting library research. Skill to critically analyze data, read protocols and apply new research techniques. Experience in following written protocols.
Advanced knowledge of and experience with pc software applications such as FlowJo, Excel, PowerPoint and Photoshop. Experience in maintaining databases. Experience using patient databases, such as EPIC.
Demonstrated experience with molecular biology techniques including RNA, PCR, DNA, CDNA, ELISA, Western Blots, SDS and agarose gel electrophoresis.
Strong organizational skills and attention to detail in order to accurately record, analyze and interpret data and patient samples.
Experience researching and gathering data and organizing information in order to produce clear and concise reports.
Familiarity with the writing of animal subjects protocols and with the surgical procedures.
Experience with laboratory equipment including centrifuges, spectrophotometers, balances, pH meters, and electrophoresis equipment. Experience to operate and maintain various lab equipment such as confocal and quantitative fluorescent microscopes, centrifuges, nanodrop and IVIS imaging machine.
Advanced theoretical knowledge of normal and malignant urologic cancer cells, stem and progenitor cell biology, and tumor microenvironment biology sufficient to aid in the design and interpretation of experiments on those topics.
Experience with peripheral blood mononuclear cell preparation and cryopreservation from clinical trial participants. Experience with surgical testicular cancer bone metastasis specimen processing and transplantation into immunodeficient mice.
- Advanced Degree (Master's, PhD).
- Employment is subject to a criminal background check.
- Willing and able to work with hazardous materials.
- Must be able to work with small animals.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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