111920 - Neural Circuit Researcher
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#111920 Neural Circuit ResearcherFiling Deadline: Tue 11/2/2021
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Department of Neurosciences laboratory using mouse genetics to define the contribution of discrete neural circuits in basic processes underlying mental health and disease.
Under supervision, incumbent will be part of a neural circuitry research laboratory focused on neural circuitry underlying mental illness including drug addiction and Parkinson's Disease. Researcher will work closely with live rodents as experimental subjects and assist in performing techniques that include molecular biology, histology, behavioral analyses, and cell culture. Performs other related functions as required.
Theoretical knowledge as acquired in a Bachelor's degree in neuroscience (or related) or two (2) years of laboratory experience in neuroscience (or related); or an equivalent combination of education and experience.
Solid organizational skills combined with proven ability to prepare and maintain clear and accurate laboratory records, files, and notebooks. Proven ability to read basic graphs and interpret and analyze data.
Excellent verbal, written, and electronic communication skills that facilitate professional, effective, and courteous interaction with a diverse population. Ability to interpret and carry out written and oral instructions effectively and accurately.
Effective interpersonal skills with strong, ability to communicate orally and in writing. Possess skills of tact, poise, diplomacy, confidentiality and flexibility in a lab environment that interacts with a diverse group of research and technical personnel and other laboratories. Ability to work in a team framework in conjunction with principles of community with other lab personnel/staff, faculty and students.
Solid computer skills using MS Word, Excel, PowerPoint, email, and a variety of web-based programs. Ability to create and maintain databases, perform standard computer tasks, learn new programs/applications with minimal direction. Commitment to update/maintain technology skills with relevant computer software.
Sensitivity to the needs of caged laboratory animals in compliance with AAALAC guidelines.
- Knowledge of brain anatomy, neural circuit function, and neurobiology.
- Experience with mouse colony management (genetics and maintenance of mouse lines) including genotyping, husbandry methods, breeding, weaning, tail clipping of offspring, basic surgical procedures, injections/euthanasia, and maintenance of water system, cage and water bottles.
- Experience performing routine histochemical staining and immunohistochemistry and troubleshooting new protocols. Experience preparing and analyzing tissue samples for histology (e.g. perfusion, tissue dissection, sectioning, immunofluorescence, microscopy).
- Experience performing molecular biology techniques including PCR, DNA preparation/extraction, PCR genotyping, immunoblotting analysis of protein, bacterial transformation, plasmid isolation, restriction digest, ligation, and Western Blot.
- Experience with general lab maintenance including maintain stocks of essential supplies and reagents, purchase lab supplies, and ensure lab is clean/organized and lab equipment is running efficiently.
- Experience working safely with potentially hazardous viral gene-transfer vectors, bacteria (for cloning) & lab chemicals. Experience making basic chemical solutions.
- Working knowledge of basic lab operations, procedures and proper use and maintenance of lab equipment. Knowledge of basic lab safety requirements as well as EH&S regulations for handling, clean-up, storage, and disposal of biohazardous substances.
- Experience using and conceptual understanding of electrophysiological, electrochemical, or imaging recordings in brain slices or in vivo physiology or behavior measurements.
- Employment is subject to a criminal background check.
- Must be willing and able to work in an animal research-related work environment.
- Must be punctual with the ability to maintain regular hours with the flexibility and willingness to work nights and weekends to assist with experiments as needed.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
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