111608 - Stem Cell Research Technician
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#111608 Stem Cell Research TechnicianFiling Deadline: Thu 9/23/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
UCSD Layoff from Career Appointment: Apply by 9/13/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 9/23/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This is a career 50% position. The hired candidate will be offered full benefits and work around 20 hours per week.
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined, and are integral to the goals of the department.
The Department has undergone significant growth in recent years with a consolidated budget of approximately $106 million including sponsored projects expenditures of approximately $54 million and clinical revenue of over $ 52 million. This expansion of the Department’s research and clinical portfolio is expected to continue in the next few years.
The Department’s 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.
In 2001, physicians and leadership of the University of California, San Diego (UCSD), Rady Children's, and Children's Specialists of San Diego (CSSD) unified pediatric patient care, research, education and community service programs, creating a university-affiliated children's health system to serve the region. Over the past two decades, an extensive list of joint programs has developed, with many physicians and researchers playing a role at both institutions.
This affiliation has consolidated the clinical, teaching, research and public service programs of the UCSD School of Medicine's Department of Pediatrics with San Diego's only health care system dedicated to the health and well-being of children. In 2009, this partnership was furthered by the creation of Rady Children’s Specialists of San Diego, a Medical Foundation, and is now recognized as having global leaders in research, technology, translational medicine, education and clinical excellence.
Assist with and perform standard laboratory procedures with Human Pluripotent Stem Cells (hESC/iPSC) and develop brain organoids as well as other experimental procedures. Incumbent will assist PIs and other lab members with studies. Studies will include: 1) General growth, expansion, maintenance, population enrichment, and induction of specific neural lines from stem cells. Induction of neural lines will involve the generation of: human astrocytes (using 3D neurosphere culture techniques), organoids, and human neurons. 2) Gene manipulation of stem cells with silencing or overexpression viral vectors constructs. 3) Characterization of resultant phenotypes (by molecular, multi-electrode array, and imaging techniques). 4) Molecular biology techniques involving the isolation of RNA and protein to quantify gene and protein expression. Procedures will include molecular biology (generation of transgene constructs, PCR, RNA isolation and quantification), histology, light microscopy of embryonic and neuronal cell lines as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, immunohistochemistry, Western blot, ELISA, tissue culture and maintenance of biochemistry equipment). Perform routine tissue culture of primary cells, preparation of lentiviruses and viral infection techniques. The candidate will be responsible for oral and written reports every other week.
Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field.
Experience with human pluripotent stem cells and neural differentiation.
Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis.
Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions.
Experience with general lab maintenance including cleaning, stocking supplies.
Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel.
Ability to follow step based experimental protocols.
Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.
Ability to prioritize and perform efficiently.
Skill and ability to keep accurate data.
Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
- Experience with UCSD policies and procedures and basic safety rules and issues.
- Experience with human pluripotent stem cells.
- Experience working in a pediatrics laboratory.
Employment is subject to a criminal background check.
Willingness to work with animals, viral vectors, radioactive material, human primary tissue and standard hazardous laboratory compounds.
Willing to work evenings and weekends when necessary.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
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