111515 - Staff Research Associate lII
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#111515 Staff Research Associate lIIFiling Deadline: Wed 10/27/2021
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Special Selection Applicants: Apply by 09/16/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Anesthesiology Research Lab was organized in 1989 to perform investigations into pharmacology and physiology of systems relevant to anesthesia and analgesia and establishes contract research in areas of developed expertise. This group has a long and active history of investigation, both preclinical and clinical, into new technologies and drug development. The strength of the group is its high level of scientific competence and its proven contract organization.
Under general supervision, the incumbent will be responsible for undertaking research projects in collaboration with the Principal Investigator (PI) and will be responsible for the day-to-day operations of the molecular biology and immunohistochemistry laboratory and cell culture facility. The Research Associate will perform histology techniques including tissue cutting (microtome and cryostat), mounting, immunohistochemical staining, and routine analysis. Incumbent will use a confocal laser scanning microscope and epi-fluorescent microscope and obtain images for analysis. Research Associate will perform Western and cell culture protocols in neural tissue and in primary and established cell lines. Incumbent will prepare study protocols, reports and data analysis using Excel/Prism or equivalent programs for grant and sponsor funded projects.
Make original and sustained contributions to several complex components of the laboratory's focus in molecular biology and advanced immunohistochemistry. Design, develop and implement complex advanced immunohistochemistry. Provide work direction to new employees, researchers, students and faculty and visiting faculty in laboratory techniques and procedures. Undertake complex research projects in collaboration with Principle Investigator.
Laboratory responsibilities include maintaining laboratory inventory and ordering, preparing solutions for laboratory support, equipment maintenance, interfacing with neighboring, laboratories and collaborators. The incumbent will provide work direction and training laboratory procedures and techniques to students, visiting scholars, and postdoctoral scholars. The incumbent reads abstracts on relevant scientific articles, finds new sources of information for novel experimental design and execution.
Advanced theoretical and practical knowledge of life science such as biology, chemistry, molecular biology, stem cell biology and biochemistry including relevant laboratory techniques as typically attained by a Bachelor’s degree, or an equivalent combination of education and experience.
Ability to perform confocal laser scanning microscopy. Theoretical knowledge and expertise of principles underlying confocal microscopy. Successful skill & ability to independently perform confocal fluorescence microscopy procedures including preparation of fluorescently stained specimens and related techniques with the skill and ability to train others.
Experience in all aspects of designing immunohistochemical methods to localize cell signaling molecules and multiple antigen labeling for fluorescent, light and confocal microscopy.
Experience in the development, interpretation, and troubleshooting of immunohistochemistry procedures.
Knowledge, experience, and ability in the development, maintenance, and execution of research protocols and techniques. Skilled in advanced immunohistochemistry techniques for the experimental design and development of new protocols. Ability to troubleshoot protocols.
Knowledge and theoretical experience in Molecular Biology or a related scientific discipline.
Experience directing and instructing researchers, faculty and students, and visiting fellows in immunohistochemical and molecular biology techniques used in the laboratory.
Excellent interpersonal, as well as written and verbal communication skills (using grammatically correct written English and accurate typing) to interact with a variety of personalities at all levels of the organization, exercising tact, mature judgment, diplomacy, and flexibility to promote positive working relationships and job effectiveness.
Experience in performing small animal perfusions for immunohistochemistry, western blotting, immunopreciptiation, PCR and cell culture in primary and established cell lines. Knowledge, experience, and demonstrated ability to procure and prepare tissues for experimental techniques.
Proven knowledge, experience and demonstrated ability in ensuring that proper safety requirements are applied, specifically Good Lab Practices and UCSD Policies and Procedures.
- Employment is subject to a criminal background check.
- Must be willing to work extended hours as required by experiments; weekend work may also be required, available to other confocal users on site, via the telephone and/or email; on call if problems arise that require immediate attention.
- Must be willing to work with hazardous materials.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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