110517 - Staff Research Assistant
#110517 Staff Research AssistantExtended Deadline: Tue 9/28/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
Job posting will remain open until position is filled.
This is a 100% limited position for one (1) year with possibility of extension or conversion to career status depending on funding availability and project needs.
Under the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of metabolic homeostasis in the nervous system by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells and mouse models.
Research project in Neurobiology & Neuroscience field involve molecular cloning strategies, tissue culture maintenance and transfection, mitochondria purification, metabolic flux analysis, biochemistry, as well as super-resolution microscopy, image analysis and data management. Incumbent will provide general lab support including ordering supplies, maintaining common lab equipment, and ensuring lab is clean, safe, and in compliance with regulations.
Theoretical and practical background and knowledge in Molecular, Cell Biology, Neurobiology / Neurosciences and Biochemistry acquired through a BS degree or higher in Biology or related field OR an equivalent combination of education and laboratory experience.
Experience with basic and general techniques in molecular biology, including PCR, gel electrophoresis, DNA cloning, and plasmid preps.
Experience in affinity purification of recombinant proteins and protein analysis (SDS-PAGE, Western blot)
Thorough knowledge of scientific terminology related to cell, molecular biology and/or biochemistry.
Knowledge and skill with sterile technique for bacterial culture. Familiarity with basic tissue culture techniques
Demonstrated experience with phase contrast and fluorescence microscopy.
Proven experience with protein purification from mammalian cell culture
Skill to prepare and maintain laboratory records, files, and notebooks. Ability to follow written and/or oral protocols.
Excellent (English) verbal, written, and electronic communication skills that facilitate professional, effective, and courteous interactions with diverse personalities. Skill to work as part of a research team.
Experience with and understanding of basic laboratory equipment (centrifuges, light microscopes, balances, pH meter, incubators, spectrophotometers, and electrophoresis apparatus)
Skill and knowledge to maintain stocks of essential supplies and reagents, to purchase lab supplies, and to ensure lab equipment is running efficiently.
Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Familiarity with Apple and PC computers and ability to work independently with a variety of computer applications such as Microsoft Office, with willingness and ability to update and increase skills and knowledge as needed. Skill to set up and maintain computerized databases of plasmid and cell line inventories.
Basic mathematical computation skills required to maintain accurate data and to prepare reagent solutions.
Well-versed with federal guidelines for Good Laboratory Practices for humane use of animals. AAALAC or AALAS certifications desirable. Knowledge of research animal care protocols and procedures as defined by AAALAC and AALAS, and governmental agencies, and sensitivity to the humane care of animals in research
- Willing and able to work safely with lab chemicals, bacteria, mouse models and human cell lines.
- Must be willing to work overtime during the evening or weekend when necessary for experiments.
- This is a 100% limited position for 1 year with possibility of extension or conversion to career status depending on funding availability and project needs.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.