110389 - Research Assistant
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.
#110389 Research AssistantExtended Deadline: Wed 10/27/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/10/flu-vaccination-requirement-for-uc-students,-faculty-and-staff-issued-for-2021-2022.html
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 07/19/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/28/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center is one of just 51 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs. The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation - from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under supervision, provide support for current scientific research projects in the lab. Assist with molecular biology and biochemistry techniques, including gel electrophoresis, DNA cloning, transformation of bacteria, plasmid sequence verifications, plasmid purification, DNA band purification from agarose gel, PCR and quantitative real-time PCR. Assist with mammalian cell culture, freezing and thawing mammalian cells, transfection of cells with plasmid DNA or siRNA. Assist with mouse husbandry, disease analysis and genotyping. Responsible for general laboratory management such as keeping accurate records of antibodies, DNA vectors, bacterial stocks and general chemical stocks, and the maintenance of a safe and clean workplace. Other duties assigned as needed.
Theoretical knowledge of biology, cell biology, molecular biology, biochemistry or related field as typically acquired through formal education at the collegiate level and/or comparable education and experience.
Ability to work with basic molecular biology techniques such as cloning, protein/DNA purification, Polymerase Chain Reaction, quantitative real time PCR, SDS-PAGE electrophoresis, and GST-pull down.
Demonstrated ability to perform routine cell culture work such as maintaining cell lines, harvesting cells for assays, treating cells, counting cells, and preparing cells for long term storage.
Ability to handle cell line and plasmid requests from other laboratories and follow proper shipment procedures for requested plasmids and cell lines (in compliance with the Codes of Federation for Transportation and IATA).
Knowledge of basic math for maintaining ledgers, income and expenditure reports. Proven skill in accurate record keeping and ability to organize, analyze and calculate data.
Proficiency in using computers for basic word processing, data entry (such as Excel), database searching (such as PubMed), and/or basic database analysis (such as BLAST). Ability to work with and learn new software for analysis.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the working environment, as well as effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Demonstrated skills, knowledge, and experience to undertake research with minimal supervision. Must have pure enthusiasm and ability for learning and genuine interest in scientific research.
Excellent organizational skills needed in order to maintain laboratory supplies and equipment, monitor laboratory safety requirements, set up and maintain records of bacterial and chemical stock.
- Knowledge of UC San Diego Environmental Health and Safety requirements and the ability to adhere to established UC San Diego guidelines for safety and environmental issues.
- Knowledge of proper UC San Diego purchasing procedures and familiarity with the UC San Diego IFIS purchasing system for ordering general lab supplies, special items, and equipment repairs.
- Employment is subject to a criminal background check.
- Must be able to work with mice.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.
UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.