110325 - Staff Research Associate
#110325 Staff Research AssociateExtended Deadline: Tue 9/21/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
UCSD Layoff from Career Appointment: Apply by 07/07/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/16/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Job posting will remain open until position is filed.
Under the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures zebrafish and mammalian cells.
Tasks involve imaging larvae and mammalian cells through microscopy (confocal and immunofluorescence), maintaining strains of mutant and transgenic zebrafish along with human cell lines, manipulation of mammalian and zebrafish gene function through genome editing, and genotyping. Responsibilities for laboratory management include tissue culture maintenance, zebrafish care, routine preparation of buffers (when appropriate), laboratory safety, ordering supplies, maintaining common lab equipment, and ensuring the lab is clean, safe, and in compliance with regulations. Will participate in the publication of scientific manuscripts based on experiments performed when appropriate.
Theoretical and practical knowledge of genetics, molecular biology, and evolution including relevant laboratory techniques, as well as aquaculture/fisheries, genetics, and/or developmental biology as typically acquired through a BS in Biology and one (1+) or more years of lab experience in any of the related fields, or equivalent combination of education and experience..
Proven ability to carry out research projects or portions of research projects with direction from PI or other experienced lab members, including (a) reading and comprehending primary scientific literature; (b) independently planning, organizing, performing, and troubleshooting experiments; (c) interpreting and presenting data.
Experience in molecular cloning, including PCR, gel electrophoresis, DNA cloning, and plasmid preps.
Demonstrated experience and ability in mammalian tissue culture
Proven knowledge and skill with sterile techniques for bacterial culture.
Proven experience with phase contrast and fluorescence microscopy.
Strong attention to detail and strong organizational skills including the ability to keep clear, accurate, and meticulous records and to interpret and follow written and/or oral protocols.
Excellent (English) verbal, written, and electronic communication skills that facilitate professional, effective, and courteous interactions with diverse personalities. Skill to work as part of a research team.
Proven experience using laboratory equipment (centrifuges, light microscopes, balances, pH meter, incubators, spectrophotometers, and electrophoresis apparatus).
Skill and knowledge to proactively maintain an organized lab and stocks of essential supplies and media, purchase new equipment, and ensure lab equipment is operating effectively.
Demonstrated knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Familiarity with Apple and PC computers. Ability to work independently with a variety of computer applications such as Microsoft Office, with ability to update and increase skills and knowledge as needed. Skill to set up and maintain computerized databases of plasmid and cell line inventories.
Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.
Ability to learn new experimental methods as required. Ability to work independently or collaboratively on a research project.
High level mathematical computation skills required to maintain accurate data and to prepare reagent solutions.
Ability to inject zebrafish embryos at the 1-cell stage with reliable and consistent results.
Strong level of practical competency in the handling, breeding, and rearing of zebrafish.
Thorough knowledge of the technical underpinnings of maintaining mutant and transgenic zebrafish strains.
- Willing and able to work safely with lab chemicals mammalian cell lines and zebrafish.
- Appropriate physical strength for handling aquaculture equipment.
- Appropriate dexterity for manipulating zebrafish embryos.
- Must be willing to be flexible with work hours to include occasional evening or weekend work as required for animal care.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
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