Job Description

Payroll Title:
PEDIATRICS/ Allergy/Immunology/Rheumatology
Salary Range
Commensurate with Experience
Appointment Type:
Appointment Percent:
RX Contract
Total Openings:
Work Schedule:
Days, 8 hrs/day, Monday - Friday

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#110218 Staff Research Associate II

Filing Deadline: Tue 9/21/2021

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit:

UCSD Layoff from Career Appointment: Apply by 7/1/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 7/13/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.


The Coufal lab studies neuroinflammation and the role of microglia, the inflammatory cell type of the brain, in neurodevelopment, aging, and both idiopathic and genetic neurologic disease using a variety of in vitro and in vivo models and stem cell derived microglia.

Under supervision of the Principal Investigator and in conjunction with postdoctoral researchers, the Staff Research Associate II will participate in a variety of different techniques to isolate nuclei from a variety of different brain types, with a goal of comparing the transcriptomic and epigenetic signature of microglia across sexes and species. These differences may underlie many neurodevelopmental and neurodegenerative diseases and identifying the conserved and divergent aspects of microglia across sex and species will help answer these questions.

Techniques utilized includes nuclei isolation, flow cytometry, and library preparation. Other lab techniques performed in the lab include induced pluripotent stem cell research, differentiation of induced pluripotent stem cells to a variety of cell fates including microglia, neurons, astrocytes, and cerebral organoids, and will learn and utilize general molecular biology experiments including: preparing RNA from cells and tissue, cDNA synthesis and quantitative PCR, performing RNAseq and ChIPseq library preparations, cloning and general molecular biology techniques, western blotting, immunohistochemistry of cells and organoids, and cryostat sectioning. There is the opportunities to learn and master a variety of techniques and to contribute not only technically but also to experimental design as able.


  • Theoretical knowledge of molecular biology or cellular biology generally acquired through a Bachelor's degree or an equivalent combination of education and experience.

  • Able to take direction, be detail oriented, follow step-wise protocols, and perform repetitive tasks in a fast-paced laboratory environment. Able to learn new procedures.

  • Ability to work independently and as part of a team, establishing and maintaining good working relationships with P.I., supervisor, postdoctoral scholars, and other members of the research group.

  • Demonstrated ability to communicate effectively in standard English, in writing, electronically, and by spoken word with lab members and vendors.

  • Ability to perform RNA purification, cDNA synthesis, and quantitative PCR assays.

  • Ability to assist lab personnel in tissue culture, including stem cell culture.

  • Knowledge of general molecular biology techniques, and use of common laboratory equipment.

  • Knowledge of laboratory safety including radioactive/biohazard handling & disposal techniques, & the ability to ensure laboratory compliance with required safety regulations.

  • Ability to troubleshoot and perform flow cytometry.


  • Experience with flow cytometry.
  • Experience with library preparation.


  • Employment is subject to a criminal background check.
  • Must be able to work in environment where biological and chemical hazards are present.
  • Strong preference to candidates with experience in flow cytometry.
  • Strong preference for candidates with experience in library preparation.
  • Must be able to work with human cells including established induced pluripotent stem cells and human embryonic stem cells.
  • Must be willing to variable work schedule to accommodate research needs, including occasional overtime, nights, weekends.
  • Must be technically detailed and careful.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!

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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see:

UC San Diego is a smoke and tobacco free environment. Please visit for more information.

Application Instructions

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