110101 - Lab Assistant II
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.
#110101 Lab Assistant IIExtended Review Date: Mon 9/20/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 07/14/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/23/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Department of Pathology at UC San Diego aims to achieve the highest standards in patient care, research and education. The Department provides diagnostic pathology services in a wide variety of subspecialties with highly trained subspecialists who are recognized for their expertise. The Department’s research programs are diverse and interface with a wealth of basic and translational research programs that are a hallmark of UC San Diego.
This Lab Assistant will work in a laboratory investigating the role of the microbiota in modulating immune responses during health and disease. Utilizing the combination of genetically engineered mice maintained under germ-free or defined microbial communities, our lab is able to explore the multifactorial contributions to disease pathogenesis. Under supervision, the applicant will assist in performing experiments in immunology and characterize mucosal immune response to gut microbes in mouse models of disease. These experiments include monitoring and treatment of laboratory animals, tissue processing for flow cytometry, histological assessment, and downstream immunological assays.
Other responsibilities include tissue culture, cell isolation and other cell-based assays, and basic cell and molecular biology techniques. Additionally, this applicant will assist in performing laboratory maintenance and administrative duties including ensuring compliance with IACUC and EH&S requirements, ordering and tracking of supplies, data organization, teaching basic laboratory techniques and preparing data for presentation.
Two (2) years of laboratory experience or two (2) years of college including courses in the natural, physical or social sciences and one (1) year of laboratory experience, or an equivalent combination of education and experience; and knowledge and abilities essential to the successful performance of the duties assigned to the position.
Theoretical knowledge in immunology or a related biomedical science.
Experience and ability to initiate and maintain mouse experiments including treatment and observation for disease. Experience with mouse handling, breeding, genotyping, anesthesia, surgeries, injections/treatments, and tissue harvest.
Proven experience and ability in multi-parameter flow cytometry (8+ colors), including panel design, staining, compensation, troubleshooting, analysis, and preparation of figures for publication.
Experience and demonstrated ability in extracting DNA/RNA, PCR, RT-PCR, qPCR, primer design, analysis and troubleshooting.
Experience and ability to initiate and maintain animal experiments multiple different eukaryotic cell lines including freezing, thawing, storage, and plating cells in preparation for experiments. Experience with sterile techniques, trouble shooting of contamination problems, and care of incubators and liquid nitrogen freezers.
Demonstrated experience in protein chemistry/purification, gel electrophoresis and transfers, various gel and membrane stains, ELISAs, western blots, and densitometry and analysis on resulting data.
Thorough knowledge of microbiological techniques, including culturing of aerobic/anaerobic bacteria, aseptic technique, and generating glycerol stocks with documentation.
Excellent communication and interpersonal skills over a wide range of situations and individuals needed to interact professionally and effectively in the work environment in person, on phone, and in writing to diverse population; including persons from foreign countries, the business community, academicians, students and staff with a high degree of diplomacy, mature judgment, tact, and flexibility.
Knowledge of proper formats, grammar, and spelling in English and proofreading ability sufficient to compose, edit, and publish professional correspondence as well as effectively and accurately document research procedures. Ability to read, comprehend, discuss, and present research materials.
Knowledge of bio, chemical, and radiation safety techniques and practices. Ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Strong organizational skills to order and maintain lab reagents and supplies, and keep accurate lab records and computer files. Experience in ordering, maintaining, trouble-shooting, and calibrating general laboratory equipment (e.g. centrifuges, incubators, microscopes, water baths, etc.).
Proficient experience with the operation of a personal computer and software applications, such as Microsoft Office (Word, Excel, Access), Photoshop, Acrobat.
Experience with reading and interpreting primary literature to obtain background knowledge in new research area and to develop new protocols or in troubleshooting assays.
- Experience with UCSD ordering, EH&S, and facilities policies and business systems.
- Employment is subject to a criminal background check.
- Must be willing to work with lab animals, gnotobiotic isolators/glove boxes, and hazardous materials.
- Must be able to work occasional evenings and weekend days, as required.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.