108136 - Laboratory Research Associate
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#108136 Laboratory Research AssociateExtended Deadline: Mon 9/27/2021
For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 03/10/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 03/19/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center is one of just 51 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation - from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Perform tumor immunology studies consisting of murine models of cancer, animal breeding and genotyping, flow cytometry, bone marrow transplantation, immunohistochemistry, in vitro cell biology, and in vitro biochemistry. Murine tumor biology studies will include xenograft, orthotopic and spontaneous tumor growth and metastasis assays, immunological analysis, treatment of animals with experimental therapeutic agents by gavage, intravenous and intraperitoneal methods, breeding and genotyping of transgenic and mutant mice, bone marrow transplantation and immune cell isolations. Perform major surgery, including small animal anesthesia. In addition, perform immunohistochemistry of frozen and fixed tissues or cells. Conduct various in vitro cell biology techniques to include performing flow cytometry analysis of cell surface protein expression, cell adhesion and migration assays, transfecting cells with expression constructs, preparing lysates of cells in culture or tissues, quantification, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations, and gel electrophoresis.
Additionally, perform recombinant DNA techniques to include preparing plasmids and maintaining plasmid stocks, analyzing DNA and RNA purification, and conducting DNA electrophoresis, and RT-PCR. Make innovative contributions regarding experimental design and methodology, using published scientific data in referred journals, as well as online using search engines and subject-specific websites, to make recommended changes to current protocols and practices. Troubleshoot problems for quality control on immunochemical and histochemical procedures. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Assume responsibility for lab maintenance and safety training. Provide training and work direction in areas of expertise as needed. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication.
Advanced theoretical knowledge in molecular biology, biochemistry, physiology and cell biology.
Demonstrated experience in mouse handling, breeding, genotyping, surgery, immunology and tumor growth and metastasis assays, including performing flow cytometry as well as immunohistochemistry.
Proven experience with immunology, cell biology, and protein biochemistry techniques; including flow cytometry, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations and gel electrophoresis, cell migrations assays, cell-substrate adhesion assays, cell-cell adhesion assays, ELISA, immunocytochemistry and cell transfection.
Experience and knowledge in molecular biology, including preparation and maintenance of virus and plasmid stocks; performing DNA and RNA purification, DNA electrophoresis and RT-PCR.
Demonstrated ability to design, develop and implement new research and experimental techniques to meet research objectives.
Experience managing laboratory environment, including ordering, safety management and maintenance of basic laboratory equipment, such as incubators, centrifuges, pipetters, spectrophotometers, balances pH meters, fluorescence microscopes, water baths, and electrophoresis equipment.
Thorough knowledge in making innovative contributions to experimental design and methodology by reviewing published scientific data in refereed journals.
Ability to accurately record experimental details and results.
Demonstrated ability use and adapt existing techniques and technologies in novel and non-traditional ways in order to meet research objectives.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials.
Ability to follow established experimental protocols with accuracy and efficiency and to design, develop and implement new research and experimental techniques to meet research objectives.
Ability to adhere to established UCSD guidelines for safety, animal care, and environmental issues.
Master's degree or PhD.
- Employment is subject to a criminal background check.
- Must be able to work occasional evenings or weekends if needed.
- Proven ability to work with animals and willingness to work with biohazardous materials.
Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
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