Job Description

Payroll Title:
SRA 3
Department:
NEUROSCIENCES
Salary Range
Commensurate with Experience
Worksite:
Campus
Appointment Type:
Career
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
2
Work Schedule:
Days, 8 hrs/day, Monday-Friday

As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.

#107699 Electron & Light Microscopy Specialist

Initial Review Date: Thu 9/23/2021

For the safety and well-being of the entire university community, the University of California requires, with few exceptions, that all students, faculty and staff be vaccinated against the COVID-19 virus before they will be allowed on campus or in a facility or office. For more information visit: https://ucnet.universityofcalifornia.edu/news/2021/07/ucs-covid-19-vaccine-policy.html

UCSD Layoff from Career Appointment: Apply by 01/28/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 02/09/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.

This position will remain open until a candidate has been successfully identified.

DESCRIPTION

The electron and light microscopy specialist collaborates with researchers from several laboratories, aids the research group and independently conducts laboratory investigations at NCMIR. Performs and develops both specimen preparation and sectioning techniques in the areas of high resolution transmission and intermediate high voltage electron microscopy, serial block-face scanning microscopy, immunofluorescence, immunocytochemistry, protein labeling, serial sectioning, computer-aided 3-dimensional reconstruction, tomographic reconstruction within thick biological specimen. Develops, modifies and implements freeze fracture techniques, high pressure freezing, 2-photon imaging and confocal microscopy.

Obtains large scale brain mapping data and examines and acquires high quality images of specimens for both data analysis and publication. Assists researchers with the processing and analysis of 3D EM data, including EM volume segmentation and morphometric analysis. Teaches and trains undergraduate students, graduate students, post-docs and collaborators on equipment and laboratory procedures used in confocal and electron microscopy.

MINIMUM QUALIFICATIONS

  • Four (4) years of related experience, education/training, OR a Bachelor's Degree in a related area. Related experience includes research in histology, histochemistry, molecular and cellular biology as well as chemistry and biochemistry.

  • Experience and skill with transmitted light microscopy, laser scanning confocal, transmission electron microscopy, and high voltage electron microscopy.

  • Experience in the production of high quality light images and electron micorgraphs employing both CCD and Direct Detection devices.

  • Experience in the production of high quality vibratome sections for confocal microscopy. Familiarity with neuroanatomical landmarks and cellular components in brain slices.

  • Experience to produce high quality thin and semi-thin sections for electron microscopy with glass and diamond knife.

  • Experience with high resolution localization labeling techniques (such as colloidal gold etc.) for electron microscopy. Ability to learn new labeling techniques.

  • Understanding both the theory and practice of various cellular localization labeling techniques for light, confocal, and transmission electron microscopy. Experience with certain cellular localization procedures. Ability to learn new labeling techniques.

  • Understanding of theory and practice of chemical tissue fixation, high pressure freeze fixation and freeze fracture for localization procedures and ultrastructural procedures used in transmission electron microscopy, confocal microscopy, and light microscopy.

  • Ability to understand and evaluate scientific literature. This includes all aspects of the research project and to interpret results for scientific publications. Ability to conduct research studies using minimal supervision.

  • Familiarity and skill with software for processing and creating two-dimensional and three-dimensional images, including use of common visualization tools (e.g., IMOD, AMIRA, etc.)

  • Skill in providing individual instruction on the use of the transmission electron microscope, confocal microscope, and peripheral support of these instruments.

  • Excellent written and oral communication skills and ability to juggle multiple projects at once with good organization skills.

PREFERRED QUALIFICATIONS

  • Knowledge of graphing software (e.g., GraphPad prism) and basic understanding of statistical analyses is a plus.
  • Basic familiarity with Linux operating system.

SPECIAL CONDITIONS

Employment is subject to a criminal background check.


Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

Application Instructions

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